Category: Cell Culture

Overview The Trypsin-EDTA method, also referred to as trypsinization, is the most commonly used method for passaging/subculturing of adherent cells….

Suspension culture is passaged by diluting the existing culture. Since cells float in the medium in suspension culture, they are not treated with a trypsin-EDTA solution. To subculture a suspension cell line, a small amount of cell suspension from the existing culture is transferred to a culture dish containing fresh growth medium.

Cell culture is not static. Cells acquire changes when maintained for a long time in culture (Masters & Stacey, 2007)….

Several methods have been developed for passaging cells. Each method has its own advantages and drawbacks. Always check the cell…

Cryopreservation is an efficient way to preserve cells at ultra-low temperatures (below -135°C) which stops all physiological processes and biological aging. It is a routinely used technique in all cell culture laboratories.

Culturing cells from multicellular organisms especially from animals (mammals, amphibians, insects, birds etc) refers to cell culture or animal cell culture.

Overview The freezing medium is used to preserve cell lines at ultra-low temperatures. This method of preserving cell lines is…

Overview Cryopreservation (Cryo: icy cold or frost; Preservation: Storage) is an efficient way of preserving cell culture at ultra-low temperatures…