Cell Culture Archives - Laboratory Notes https://www.laboratorynotes.com/category/lab-notes-biotechniques/cell-culture/ Sun, 16 Aug 2026 22:07:49 +0000 en hourly 1 https://wordpress.org/?v=7.1.1 Trehalose as Cryoprotectant https://www.laboratorynotes.com/trehalose-as-cryoprotectant/ https://www.laboratorynotes.com/trehalose-as-cryoprotectant/#respond Sat, 08 Aug 2026 08:56:38 +0000 https://www.laboratorynotes.com/?p=28905 Trehalose is a non‑permeating cryoprotectant that stabilizes membranes, protects proteins, and reduces ice formation. This article explains its mechanisms and applications in cryobiology.

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]]> https://www.laboratorynotes.com/trehalose-as-cryoprotectant/feed/ 0 Glass Transition Temperature https://www.laboratorynotes.com/glass-transition-temperature/ https://www.laboratorynotes.com/glass-transition-temperature/#respond Thu, 06 Aug 2026 18:58:06 +0000 https://www.laboratorynotes.com/?p=28821 The glass transition temperature (Tg) marks the point where biological solutions become glass‑like and molecular motion stops. This article explains Tg, its mechanism, and its importance in cryopreservation.

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]]> https://www.laboratorynotes.com/glass-transition-temperature/feed/ 0 ITS Liquid Media Supplement https://www.laboratorynotes.com/its-liquid-media-supplement/ https://www.laboratorynotes.com/its-liquid-media-supplement/#respond Wed, 05 Aug 2026 05:25:04 +0000 https://www.laboratorynotes.com/?p=28736 ITS Liquid Media Supplement provides insulin, transferrin, and selenium to support serum‑free cell culture, metabolic stability, and high‑viability growth. This article explains its composition, biological function, and major suppliers.

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]]> https://www.laboratorynotes.com/its-liquid-media-supplement/feed/ 0 Sterile Liquid Handling: Pipetting and Dispensing Using a Micropipette  https://www.laboratorynotes.com/sterile-liquid-handling-pipetting-and-dispensing-using-a-micropipette/ https://www.laboratorynotes.com/sterile-liquid-handling-pipetting-and-dispensing-using-a-micropipette/#respond Sat, 01 Aug 2026 17:12:44 +0000 https://www.laboratorynotes.com/?p=28407 Sterile liquid handling ensures accurate, contamination‑free transfer of samples in microbiology, cell culture, and pharmaceutical work. This article explains essential pipetting and dispensing techniques used to maintain sterility and precision in laboratory environments.

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]]> https://www.laboratorynotes.com/sterile-liquid-handling-pipetting-and-dispensing-using-a-micropipette/feed/ 0 Applications of Immortalized Cell Lines https://www.laboratorynotes.com/applications-of-immortalized-cell-lines/ https://www.laboratorynotes.com/applications-of-immortalized-cell-lines/#respond Mon, 11 Aug 2025 07:44:26 +0000 https://www.laboratorynotes.com/?p=23318 The post Applications of Immortalized Cell Lines appeared first on Laboratory Notes.

]]> https://www.laboratorynotes.com/applications-of-immortalized-cell-lines/feed/ 0 Immortalized Cell Lines as Alternatives to Primary Cultures https://www.laboratorynotes.com/immortalized-cell-lines-as-alternatives-to-primary-cultures/ https://www.laboratorynotes.com/immortalized-cell-lines-as-alternatives-to-primary-cultures/#respond Mon, 30 Jun 2025 15:44:22 +0000 https://www.laboratorynotes.com/?p=21138 The post Immortalized Cell Lines as Alternatives to Primary Cultures appeared first on Laboratory Notes.

]]> https://www.laboratorynotes.com/immortalized-cell-lines-as-alternatives-to-primary-cultures/feed/ 0 Challenges in Primary Tissue Processing for Cell Isolation https://www.laboratorynotes.com/challenges-in-primary-tissue-processing-for-cell-isolation/ https://www.laboratorynotes.com/challenges-in-primary-tissue-processing-for-cell-isolation/#respond Mon, 30 Jun 2025 15:16:37 +0000 https://www.laboratorynotes.com/?p=21136 The post Challenges in Primary Tissue Processing for Cell Isolation appeared first on Laboratory Notes.

]]> https://www.laboratorynotes.com/challenges-in-primary-tissue-processing-for-cell-isolation/feed/ 0 Differences in Gene Expression Between Subconfluent and Confluent Cells https://www.laboratorynotes.com/differences-in-gene-expression-between-subconfluent-and-confluent-cells/ https://www.laboratorynotes.com/differences-in-gene-expression-between-subconfluent-and-confluent-cells/#respond Sun, 22 Jun 2025 17:10:14 +0000 https://www.laboratorynotes.com/?p=20315 The post Differences in Gene Expression Between Subconfluent and Confluent Cells appeared first on Laboratory Notes.

]]> https://www.laboratorynotes.com/differences-in-gene-expression-between-subconfluent-and-confluent-cells/feed/ 0 Fetal Bovine Serum (FBS) Alternatives in Cell Culture https://www.laboratorynotes.com/fetal-bovine-serum-fbs-alternatives-in-cell-culture/ https://www.laboratorynotes.com/fetal-bovine-serum-fbs-alternatives-in-cell-culture/#respond Sun, 22 Jun 2025 17:02:36 +0000 https://www.laboratorynotes.com/?p=20313 The post Fetal Bovine Serum (FBS) Alternatives in Cell Culture appeared first on Laboratory Notes.

]]> https://www.laboratorynotes.com/fetal-bovine-serum-fbs-alternatives-in-cell-culture/feed/ 0 Human Platelet Lysate https://www.laboratorynotes.com/human-platelet-lysate/ https://www.laboratorynotes.com/human-platelet-lysate/#respond Sun, 22 Jun 2025 16:49:54 +0000 https://www.laboratorynotes.com/?p=20309 The post Human Platelet Lysate appeared first on Laboratory Notes.

]]> https://www.laboratorynotes.com/human-platelet-lysate/feed/ 0 Fetal Bovine Serum (FBS) Composition https://www.laboratorynotes.com/fetal-bovine-serum-fbs-composition/ https://www.laboratorynotes.com/fetal-bovine-serum-fbs-composition/#respond Sun, 22 Jun 2025 11:07:20 +0000 https://www.laboratorynotes.com/?p=20294 The post Fetal Bovine Serum (FBS) Composition appeared first on Laboratory Notes.

]]> https://www.laboratorynotes.com/fetal-bovine-serum-fbs-composition/feed/ 0 Circadian Gene Expression in Cultured Cells https://www.laboratorynotes.com/circadian-gene-expression-in-cultured-cells/ https://www.laboratorynotes.com/circadian-gene-expression-in-cultured-cells/#respond Sun, 22 Jun 2025 10:56:07 +0000 https://www.laboratorynotes.com/?p=20290 The post Circadian Gene Expression in Cultured Cells appeared first on Laboratory Notes.

]]> https://www.laboratorynotes.com/circadian-gene-expression-in-cultured-cells/feed/ 0 Fetal Bovine Serum (FBS) https://www.laboratorynotes.com/fetal-bovine-serum-fbs/ https://www.laboratorynotes.com/fetal-bovine-serum-fbs/#respond Sun, 22 Jun 2025 06:20:50 +0000 https://www.laboratorynotes.com/?p=20285 The post Fetal Bovine Serum (FBS) appeared first on Laboratory Notes.

]]> https://www.laboratorynotes.com/fetal-bovine-serum-fbs/feed/ 0 Impact of Amphotericin B on Mitochondria in Mammalian Cell Cultures https://www.laboratorynotes.com/impact-of-amphotericin-b-on-mitochondria-in-mammalian-cell-cultures/ https://www.laboratorynotes.com/impact-of-amphotericin-b-on-mitochondria-in-mammalian-cell-cultures/#respond Tue, 17 Jun 2025 00:20:07 +0000 https://www.laboratorynotes.com/?p=20096 The post Impact of Amphotericin B on Mitochondria in Mammalian Cell Cultures appeared first on Laboratory Notes.

]]> https://www.laboratorynotes.com/impact-of-amphotericin-b-on-mitochondria-in-mammalian-cell-cultures/feed/ 0 Amphotericin B in Cell Culture https://www.laboratorynotes.com/amphotericin-b-in-cell-culture/ https://www.laboratorynotes.com/amphotericin-b-in-cell-culture/#respond Tue, 17 Jun 2025 00:14:23 +0000 https://www.laboratorynotes.com/?p=20094 The post Amphotericin B in Cell Culture appeared first on Laboratory Notes.

]]> https://www.laboratorynotes.com/amphotericin-b-in-cell-culture/feed/ 0 Impact of Kanamycin on Mitochondria in Mammalian Cell Cultures https://www.laboratorynotes.com/impact-of-kanamycin-on-mitochondria-in-mammalian-cell-cultures/ https://www.laboratorynotes.com/impact-of-kanamycin-on-mitochondria-in-mammalian-cell-cultures/#respond Tue, 17 Jun 2025 00:02:02 +0000 https://www.laboratorynotes.com/?p=20092 The post Impact of Kanamycin on Mitochondria in Mammalian Cell Cultures appeared first on Laboratory Notes.

]]> https://www.laboratorynotes.com/impact-of-kanamycin-on-mitochondria-in-mammalian-cell-cultures/feed/ 0 Kanamycin in Cell Culture https://www.laboratorynotes.com/kanamycin-in-cell-culture/ https://www.laboratorynotes.com/kanamycin-in-cell-culture/#respond Mon, 16 Jun 2025 22:26:47 +0000 https://www.laboratorynotes.com/?p=20090 The post Kanamycin in Cell Culture appeared first on Laboratory Notes.

]]> https://www.laboratorynotes.com/kanamycin-in-cell-culture/feed/ 0 Impact of Gentamicin on Mitochondria in Mammalian Cell Cultures https://www.laboratorynotes.com/impact-of-gentamicin-on-mitochondria-in-mammalian-cell-cultures/ https://www.laboratorynotes.com/impact-of-gentamicin-on-mitochondria-in-mammalian-cell-cultures/#respond Mon, 16 Jun 2025 21:47:34 +0000 https://www.laboratorynotes.com/?p=20079 Gentamicin, like other aminoglycoside antibiotics, primarily targets bacterial ribosomes, binding to the 30S subunit to disrupt protein synthesis and cause...

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]]> https://www.laboratorynotes.com/impact-of-gentamicin-on-mitochondria-in-mammalian-cell-cultures/feed/ 0 Gentamicin in Cell Culture https://www.laboratorynotes.com/gentamicin-in-cell-culture/ https://www.laboratorynotes.com/gentamicin-in-cell-culture/#respond Mon, 16 Jun 2025 16:39:49 +0000 https://www.laboratorynotes.com/?p=20072 The post Gentamicin in Cell Culture appeared first on Laboratory Notes.

]]> https://www.laboratorynotes.com/gentamicin-in-cell-culture/feed/ 0 Impact of Streptomycin on Mitochondria in Mammalian Cell Cultures https://www.laboratorynotes.com/impact-of-streptomycin-on-mitochondria-in-mammalian-cell-cultures/ https://www.laboratorynotes.com/impact-of-streptomycin-on-mitochondria-in-mammalian-cell-cultures/#respond Mon, 16 Jun 2025 16:23:48 +0000 https://www.laboratorynotes.com/?p=20070 The post Impact of Streptomycin on Mitochondria in Mammalian Cell Cultures appeared first on Laboratory Notes.

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