Lab Notes: Biotechniques Archives - Laboratory Notes https://www.laboratorynotes.com/category/lab-notes-biotechniques/ Sun, 09 Aug 2026 09:47:35 +0000 en hourly 1 https://wordpress.org/?v=7.0.3 Trehalose as Cryoprotectant https://www.laboratorynotes.com/trehalose-as-cryoprotectant/ https://www.laboratorynotes.com/trehalose-as-cryoprotectant/#respond Sat, 08 Aug 2026 08:56:38 +0000 https://www.laboratorynotes.com/?p=28905 Trehalose is a non‑permeating cryoprotectant that stabilizes membranes, protects proteins, and reduces ice formation. This article explains its mechanisms and applications in cryobiology.

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]]> https://www.laboratorynotes.com/trehalose-as-cryoprotectant/feed/ 0 Glass Transition Temperature https://www.laboratorynotes.com/glass-transition-temperature/ https://www.laboratorynotes.com/glass-transition-temperature/#respond Thu, 06 Aug 2026 18:58:06 +0000 https://www.laboratorynotes.com/?p=28821 The glass transition temperature (Tg) marks the point where biological solutions become glass‑like and molecular motion stops. This article explains Tg, its mechanism, and its importance in cryopreservation.

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]]> https://www.laboratorynotes.com/glass-transition-temperature/feed/ 0 ITS Liquid Media Supplement https://www.laboratorynotes.com/its-liquid-media-supplement/ https://www.laboratorynotes.com/its-liquid-media-supplement/#respond Wed, 05 Aug 2026 05:25:04 +0000 https://www.laboratorynotes.com/?p=28736 ITS Liquid Media Supplement provides insulin, transferrin, and selenium to support serum‑free cell culture, metabolic stability, and high‑viability growth. This article explains its composition, biological function, and major suppliers.

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]]> https://www.laboratorynotes.com/its-liquid-media-supplement/feed/ 0 Sterile Liquid Handling: Pipetting and Dispensing Using a Micropipette  https://www.laboratorynotes.com/sterile-liquid-handling-pipetting-and-dispensing-using-a-micropipette/ https://www.laboratorynotes.com/sterile-liquid-handling-pipetting-and-dispensing-using-a-micropipette/#respond Sat, 01 Aug 2026 17:12:44 +0000 https://www.laboratorynotes.com/?p=28407 Sterile liquid handling ensures accurate, contamination‑free transfer of samples in microbiology, cell culture, and pharmaceutical work. This article explains essential pipetting and dispensing techniques used to maintain sterility and precision in laboratory environments.

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]]> https://www.laboratorynotes.com/sterile-liquid-handling-pipetting-and-dispensing-using-a-micropipette/feed/ 0 TensorFlow https://www.laboratorynotes.com/tensorflow/ https://www.laboratorynotes.com/tensorflow/#respond Fri, 10 Oct 2025 17:19:50 +0000 https://www.laboratorynotes.com/?p=26737 TensorFlow is an open-source software library developed by the Google Brain Team for numerical computation and large-scale machine learning. It...

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]]> https://www.laboratorynotes.com/tensorflow/feed/ 0 Keras https://www.laboratorynotes.com/keras/ https://www.laboratorynotes.com/keras/#respond Fri, 10 Oct 2025 17:17:17 +0000 https://www.laboratorynotes.com/?p=26735 Keras is an open-source, high-level neural network library designed to simplify the creation, training, and deployment of deep learning models....

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]]> https://www.laboratorynotes.com/keras/feed/ 0 PyTorch https://www.laboratorynotes.com/pytorch/ https://www.laboratorynotes.com/pytorch/#respond Fri, 10 Oct 2025 17:14:02 +0000 https://www.laboratorynotes.com/?p=26733 PyTorch is an open-source deep learning framework developed by Facebook’s AI Research Lab (FAIR) and officially released in 2016. It...

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]]> https://www.laboratorynotes.com/pytorch/feed/ 0 DNA Microarray https://www.laboratorynotes.com/dna-microarray/ https://www.laboratorynotes.com/dna-microarray/#respond Mon, 06 Oct 2025 23:45:41 +0000 https://www.laboratorynotes.com/?p=26728 A DNA microarray is a powerful molecular biology tool used to measure the expression levels of thousands of genes simultaneously...

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]]> https://www.laboratorynotes.com/dna-microarray/feed/ 0 Mechanism of Chain Termination in Sanger Sequencing https://www.laboratorynotes.com/mechanism-of-chain-termination-in-sanger-sequencing/ https://www.laboratorynotes.com/mechanism-of-chain-termination-in-sanger-sequencing/#respond Fri, 03 Oct 2025 10:19:44 +0000 https://www.laboratorynotes.com/?p=26701 In Sanger sequencing, a mixture of normal deoxyribonucleotides (dNTPs) and modified dideoxyribonucleotides (ddNTPs) is used to control DNA chain elongation. ...

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]]> https://www.laboratorynotes.com/mechanism-of-chain-termination-in-sanger-sequencing/feed/ 0 Sanger Sequencing: Historical Foundations to Modern Refinements https://www.laboratorynotes.com/sanger-sequencing-historical-foundations-to-modern-refinements/ https://www.laboratorynotes.com/sanger-sequencing-historical-foundations-to-modern-refinements/#respond Thu, 02 Oct 2025 21:09:50 +0000 https://www.laboratorynotes.com/?p=26685 Sanger Sequencing, also known as the chain-termination method, stands as one of the most significant breakthroughs in molecular biology. Developed...

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]]> https://www.laboratorynotes.com/sanger-sequencing-historical-foundations-to-modern-refinements/feed/ 0 Sanger Sequencing Vs Next-Generation Sequencing https://www.laboratorynotes.com/sanger-sequencing-vs-next-generation-sequencing/ https://www.laboratorynotes.com/sanger-sequencing-vs-next-generation-sequencing/#respond Mon, 29 Sep 2025 12:52:01 +0000 https://www.laboratorynotes.com/?p=26639 Criteria Sanger Sequencing Next-Generation Sequencing (NGS) Remarks Definition A first-generation DNA sequencing method based on selective incorporation of chain-terminating dideoxynucleotides...

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]]> https://www.laboratorynotes.com/sanger-sequencing-vs-next-generation-sequencing/feed/ 0 High-Throughput Genomic Sequencing Technology https://www.laboratorynotes.com/high-throughput-genomic-sequencing-technology/ https://www.laboratorynotes.com/high-throughput-genomic-sequencing-technology/#comments Mon, 29 Sep 2025 06:54:30 +0000 https://www.laboratorynotes.com/?p=26634 High-throughput genomic sequencing technologies, often referred to as next-generation sequencing (NGS), represent a transformative advancement in molecular biology and medicine,...

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]]> https://www.laboratorynotes.com/high-throughput-genomic-sequencing-technology/feed/ 1 Sequencing https://www.laboratorynotes.com/sequencing/ https://www.laboratorynotes.com/sequencing/#respond Sat, 27 Sep 2025 19:43:12 +0000 https://www.laboratorynotes.com/?p=26562 Sequencing refers to the experimental process of determining the precise order of the building blocks that make up a biological...

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]]> https://www.laboratorynotes.com/sequencing/feed/ 0 Common Substrates of Horseradish Peroxidase https://www.laboratorynotes.com/common-substrates-of-horseradish-peroxidase/ https://www.laboratorynotes.com/common-substrates-of-horseradish-peroxidase/#respond Mon, 22 Sep 2025 13:25:40 +0000 https://www.laboratorynotes.com/?p=26097 Horseradish peroxidase (HRP) is widely used in immunoassays and histological techniques because of its ability to catalyze the oxidation of...

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]]> https://www.laboratorynotes.com/common-substrates-of-horseradish-peroxidase/feed/ 0 Immunohistochemistry https://www.laboratorynotes.com/immunohistochemistry/ https://www.laboratorynotes.com/immunohistochemistry/#respond Mon, 22 Sep 2025 08:41:33 +0000 https://www.laboratorynotes.com/?p=25838 Immunohistochemistry (IHC) is a widely used laboratory technique that combines histology, immunology, and chemistry to detect and visualize the presence...

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]]> https://www.laboratorynotes.com/immunohistochemistry/feed/ 0 Antigen Retrieval https://www.laboratorynotes.com/antigen-retrieval/ https://www.laboratorynotes.com/antigen-retrieval/#respond Mon, 22 Sep 2025 07:33:54 +0000 https://www.laboratorynotes.com/?p=25836 Antigen retrieval is a crucial laboratory technique used in immunohistochemistry (IHC) and related staining methods to unmask antigenic sites (epitopes)...

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]]> https://www.laboratorynotes.com/antigen-retrieval/feed/ 0 Electron Microscopy https://www.laboratorynotes.com/electron-microscopy/ https://www.laboratorynotes.com/electron-microscopy/#respond Sat, 20 Sep 2025 19:08:20 +0000 https://www.laboratorynotes.com/?p=25823 Electron microscopy (EM) is a powerful imaging technique that uses a beam of electrons instead of visible light to visualize...

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]]> https://www.laboratorynotes.com/electron-microscopy/feed/ 0 Correlative NanoSIMS https://www.laboratorynotes.com/correlative-nanosims/ https://www.laboratorynotes.com/correlative-nanosims/#respond Sat, 20 Sep 2025 18:58:14 +0000 https://www.laboratorynotes.com/?p=25821 Correlative NanoSIMS is an advanced analytical approach that integrates nanoscale secondary ion mass spectrometry (NanoSIMS) with complementary imaging and analytical...

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]]> https://www.laboratorynotes.com/correlative-nanosims/feed/ 0 Nanoscale Secondary Ion Mass Spectrometry (NanoSIMS) https://www.laboratorynotes.com/nanoscale-secondary-ion-mass-spectrometry-nanosims/ https://www.laboratorynotes.com/nanoscale-secondary-ion-mass-spectrometry-nanosims/#respond Sat, 20 Sep 2025 18:16:05 +0000 https://www.laboratorynotes.com/?p=25809 Nanoscale Secondary Ion Mass Spectrometry (NanoSIMS) is an advanced surface analysis technique that provides high-resolution chemical and isotopic imaging at...

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]]> https://www.laboratorynotes.com/nanoscale-secondary-ion-mass-spectrometry-nanosims/feed/ 0 Time-of-Flight Secondary Ion Mass Spectrometry (TOF-SIMS) https://www.laboratorynotes.com/time-of-flight-secondary-ion-mass-spectrometry-tof-sims/ https://www.laboratorynotes.com/time-of-flight-secondary-ion-mass-spectrometry-tof-sims/#respond Sat, 20 Sep 2025 18:10:10 +0000 https://www.laboratorynotes.com/?p=25807 Time-of-Flight Secondary Ion Mass Spectrometry (TOF-SIMS) is a highly sensitive surface analysis technique that provides detailed chemical and molecular information...

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]]> https://www.laboratorynotes.com/time-of-flight-secondary-ion-mass-spectrometry-tof-sims/feed/ 0