Lab Notes: Molecular Cloning Archives - Laboratory Notes https://www.laboratorynotes.com/category/lab-notes-molecular-biology/lab-notes-molecular-cloning/ Tue, 06 May 2025 07:15:27 +0000 en hourly 1 https://wordpress.org/?v=6.9 Gibson Assembly https://www.laboratorynotes.com/gibson-assembly/ https://www.laboratorynotes.com/gibson-assembly/#respond Tue, 06 May 2025 07:15:26 +0000 https://www.laboratorynotes.com/?p=18810 Gibson Assembly is an efficient molecular cloning method that allows seamless assembly of multiple DNA fragments in a single reaction....

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]]> https://www.laboratorynotes.com/gibson-assembly/feed/ 0 Molecular Cloning https://www.laboratorynotes.com/molecular-cloning/ https://www.laboratorynotes.com/molecular-cloning/#respond Tue, 06 May 2025 05:40:15 +0000 https://www.laboratorynotes.com/?p=18800 Molecular cloning is a fundamental technique in molecular biology that enables the creation of identical DNA copies and their propagation...

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]]> https://www.laboratorynotes.com/molecular-cloning/feed/ 0 dCas9 (Endonuclease Dead Cas9) https://www.laboratorynotes.com/dcas9-endonuclease-dead-cas9/ https://www.laboratorynotes.com/dcas9-endonuclease-dead-cas9/#respond Thu, 11 May 2023 03:00:53 +0000 https://www.laboratorynotes.com/?p=13640 dCas9 is a mutated version of Cas 9 that lacks endonuclease activity (Endonuclease dead Cas9). It is created by point...

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]]> https://www.laboratorynotes.com/dcas9-endonuclease-dead-cas9/feed/ 0 Targeting RNA using CRISPR-Cas https://www.laboratorynotes.com/targeting-rna-using-crispr-cas/ https://www.laboratorynotes.com/targeting-rna-using-crispr-cas/#respond Sat, 06 May 2023 06:02:50 +0000 https://www.laboratorynotes.com/?p=13664 The following CRISPR-Cas systems can be used to target and modify RNA in cells. The following Cas proteins can recognize...

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