electrophoresis buffer Archives - Laboratory Notes

TBE (Tris-Borate-EDTA) Electrophoresis Buffer Calculator

admin — Wed, 29 Sep 2021 06:42:34 +0000

The TBE (Tris-Borate-EDTA) electrophoresis buffer contains Tris base, Boric acid, EDTA and has pH 8.3. Usually the stock TBE electrophoresis...

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Preparation of 10x MOPS Electrophoresis Buffer

admin — Thu, 09 Sep 2021 18:26:09 +0000

OVERVIEW MOPS is a zwitterionic physiological buffer, with pH range 6.5 – 7.9. MOPS-containing electrophoresis buffer is suitable for RNA...

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Preparation of 50X TAE Electrophoresis Buffer

admin — Sat, 12 Sep 2020 08:11:47 +0000

A 50x TAE buffer can be prepared by mixing and dissolving 242 g Tris base, 100 ml of 0.5 M EDTA and 57.1 ml glacial acetic acid in a deionized water to a final volume of 1000 ml. The pH of the final solution should be between 8.2 - 8.4.

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Preparation of 5X TBE Electrophoresis Buffer

admin — Sat, 12 Sep 2020 05:49:36 +0000

TBE is a very common electrophoresis buffer for DNA agarose gel electrophoresis. It is prepared as a 5X stock solution that can be diluted to 1x or 0.5x working solution for DNA agarose gel electrophoresis. The 5X concentrated solution contains 0.445 M Tris borate and 0.01 M EDTA (pH 8.2 – 8.4).

A 5X stock solution is prepared by dissolving 54 g Tris base, 27.5 g Boric acid, and 20 ml of 0.5 M EDTA in water to a final volume of 1000 ml. The solution is sterilized by autoclaving and is stored at room temperature.

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