plasmid Archives - Laboratory Notes

Plasmidome

admin — Sat, 19 Apr 2025 22:42:22 +0000

The plasmidome represents the complete plasmid content within a microbial community or environment, encompassing the collective genetic information carried by...

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pEC156

admin — Fri, 18 Apr 2025 23:37:29 +0000

pEC156 is a naturally occurring plasmid found in Escherichia coli, classified as a small, high-copy-number, non-conjugative but mobilizable plasmid. With...

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Factors Influencing Plasmid Host-Range Specificity in Bacteria

admin — Fri, 18 Apr 2025 13:20:41 +0000

The host-range specificity of a plasmid refers to the spectrum of bacterial species in which the plasmid can successfully replicate...

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Plasmid: Geometry and Conformation

admin — Fri, 18 Apr 2025 07:47:38 +0000

Plasmid geometry and conformation represent fundamental aspects of plasmid structure that influence their biological function and behavior in both natural...

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Plasmid DNA Isolation by the Alkaline Lysis Method Vs Boiling Lysis Method

admin — Mon, 14 Apr 2025 04:28:47 +0000

Criteria Alkaline Lysis Method Boiling Lysis Method Remarks Principle Selective chemical denaturation using alkaline pH and detergent to separate plasmid...

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Plasmid DNA Vs Chromosomal DNA

admin — Sun, 13 Apr 2025 01:46:18 +0000

Criteria Plasmid DNA Chromosomal DNA Remarks Definition Small, circular, double-stranded extrachromosomal DNA molecule found mainly in bacteria and some eukaryotes...

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2µ Plasmid

admin — Sun, 13 Apr 2025 01:22:47 +0000

The 2µ plasmid, also called 2-µm plasmid, is a naturally occurring, circular DNA molecule found in Saccharomyces cerevisiae (budding yeast). ...

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Ti Plasmid

admin — Sun, 13 Apr 2025 01:11:24 +0000

The Ti plasmid, or tumor-inducing plasmid, is an extrachromosomal DNA molecule found naturally in Agrobacterium tumefaciens. This bacterium is infamous...

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Natural Hosts of Plasmids Across Life Domains

admin — Sun, 13 Apr 2025 01:00:46 +0000

Plasmids occur naturally in a variety of hosts, primarily bacteria but also archaea and eukaryotes. In bacteria, plasmids are often...

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Chloramphenicol Amplification of Low-copy Number Plasmids

admin — Sun, 26 Mar 2023 19:05:43 +0000

Low-copy number plasmid vectors such as those based on pMB1 or ColE1 can be amplified by adding chloramphenicol to the...

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Plasmid: pBR322 (Cloning Vector)

admin — Sun, 26 Mar 2023 19:05:25 +0000

Plasmid name pBR322 Application Cloning vector Replicon pMBI Host E. coli DH5α, and other related strains Plasmid size (kbp) 4.361...

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Plasmid Isolation: Genomic DNA vs Plasmid DNA

admin — Sun, 26 Mar 2023 19:05:23 +0000

One of the major challenges in plasmid isolation methods is the separation of plasmid DNA from genomic DNA. To better...

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Plasmid: pSymA

admin — Sun, 26 Mar 2023 06:20:49 +0000

Plasmid name pSymA Host Sinorhizobium meliloti Plasmid size(kbp) 1354.2 Plasmid geometry Circular Plasmid copy number 2–3

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Plasmid: SCP1

admin — Sun, 26 Mar 2023 06:20:48 +0000

Plasmid name SCP1 Host Streptomyces coelicolor Plasmid size(kbp) 350.0 Plasmid geometry Linear Plasmid copy number 4

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Plasmid: F

admin — Sun, 26 Mar 2023 06:20:47 +0000

Plasmid name F Host Escherichia coli Plasmid size(kbp) 99.2 Plasmid geometry Circular Plasmid copy number 1–2

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Plasmid: lp25

admin — Sun, 26 Mar 2023 06:20:43 +0000

Plasmid name lp25 Host Borrelia burgdorferi Plasmid size(kbp) 24.2 Plasmid geometry Linear Plasmid copy number 1–2

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Plasmid: ColE1

admin — Sat, 25 Mar 2023 19:05:02 +0000

Plasmid name ColE1 Host Escherichia coli Plasmid size(kbp) 6.6 Plasmid geometry Circular Plasmid copy number 10–30

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Plasmid: pUB110

admin — Sat, 25 Mar 2023 19:05:00 +0000

Plasmid name pUB110 Host Bacillus subtilis Plasmid size(kbp) 2.3 Plasmid geometry Circular Plasmid copy number 20–50

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Plasmid: Timeline of Key Events

admin — Mon, 20 Mar 2023 16:45:56 +0000

1903: The chromosome theory of inheritance was developed independently by two researchers: Walter S. Sutton and Theodor Boveri 1910: The...

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Plasmid vs Virus

admin — Mon, 20 Mar 2023 16:45:42 +0000

Criteria Plasmid Virus Remarks Definition Small, circular or linear extrachromosomal DNA molecules found in bacteria and some eukaryotes Infectious acellular...

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