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	<title>Solution Archives - Laboratory Notes</title>
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		<title>Preparation of Potassium Chloride-based 10x PCR Buffer</title>
		<link>https://www.laboratorynotes.com/preparation-of-potassium-chloride-based-10x-pcr-buffer/</link>
					<comments>https://www.laboratorynotes.com/preparation-of-potassium-chloride-based-10x-pcr-buffer/#respond</comments>
		
		<dc:creator><![CDATA[admin]]></dc:creator>
		<pubDate>Fri, 24 Mar 2023 05:10:32 +0000</pubDate>
				<category><![CDATA[Reagents]]></category>
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		<guid isPermaLink="false">https://www.laboratorynotes.com/?p=11757</guid>

					<description><![CDATA[<p>OVERVIEWHere we describe a procedure to prepare 10 ml of Potassium chloride-based 10x PCR buffer. The 10x buffer contains 500...</p>
<p>The post <a href="https://www.laboratorynotes.com/preparation-of-potassium-chloride-based-10x-pcr-buffer/">Preparation of Potassium Chloride-based 10x PCR Buffer</a> appeared first on <a href="https://www.laboratorynotes.com">Laboratory Notes</a>.</p>
]]></description>
		
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		<title>Preparation of 0.1 mg/ml Poly-D-Lysine Solution from Stock Solution</title>
		<link>https://www.laboratorynotes.com/preparation-of-0-1mg-ml-poly-d-lysine-solution-from-stock-solution/</link>
					<comments>https://www.laboratorynotes.com/preparation-of-0-1mg-ml-poly-d-lysine-solution-from-stock-solution/#respond</comments>
		
		<dc:creator><![CDATA[admin]]></dc:creator>
		<pubDate>Sat, 21 Jan 2023 18:59:55 +0000</pubDate>
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		<guid isPermaLink="false">https://www.laboratorynotes.com/?p=11277</guid>

					<description><![CDATA[<p>OVERVIEWA 0.1 mg/ml Poly-d-lysine solution is used to coat coverslips. Coated coverslips allow cells to adhere and grow. A 0.1...</p>
<p>The post <a href="https://www.laboratorynotes.com/preparation-of-0-1mg-ml-poly-d-lysine-solution-from-stock-solution/">Preparation of 0.1 mg/ml Poly-D-Lysine Solution from Stock Solution</a> appeared first on <a href="https://www.laboratorynotes.com">Laboratory Notes</a>.</p>
]]></description>
		
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		<title>Preparation of 4% Paraformaldehyde Solution in PBS from 16% Stock Solution</title>
		<link>https://www.laboratorynotes.com/preparation-of-4-paraformaldehyde-solution-in-pbs-from-16-stock-solution/</link>
					<comments>https://www.laboratorynotes.com/preparation-of-4-paraformaldehyde-solution-in-pbs-from-16-stock-solution/#respond</comments>
		
		<dc:creator><![CDATA[admin]]></dc:creator>
		<pubDate>Sat, 14 Jan 2023 14:19:30 +0000</pubDate>
				<category><![CDATA[Reagents]]></category>
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		<guid isPermaLink="false">https://www.laboratorynotes.com/?p=11170</guid>

					<description><![CDATA[<p>OVERVIEW A 4% Paraformaldehyde Solution can be prepared by diluting a concentrated 16% paraformaldehyde stock solution. To prepare 100 ml...</p>
<p>The post <a href="https://www.laboratorynotes.com/preparation-of-4-paraformaldehyde-solution-in-pbs-from-16-stock-solution/">Preparation of 4% Paraformaldehyde Solution in PBS from 16% Stock Solution</a> appeared first on <a href="https://www.laboratorynotes.com">Laboratory Notes</a>.</p>
]]></description>
		
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		<title>Preparation of 50% Ethanol from 95% Ethanol</title>
		<link>https://www.laboratorynotes.com/preparation-of-50-ethanol-from-95-ethanol/</link>
					<comments>https://www.laboratorynotes.com/preparation-of-50-ethanol-from-95-ethanol/#respond</comments>
		
		<dc:creator><![CDATA[admin]]></dc:creator>
		<pubDate>Thu, 12 Jan 2023 10:03:28 +0000</pubDate>
				<category><![CDATA[Reagents]]></category>
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		<guid isPermaLink="false">https://www.laboratorynotes.com/?p=11072</guid>

					<description><![CDATA[<p>OVERVIEWA 1L aqueous solution of 50% Ethanol can be prepared by diluting 95% ethanol. To prepare 1L of 50% ethanol,...</p>
<p>The post <a href="https://www.laboratorynotes.com/preparation-of-50-ethanol-from-95-ethanol/">Preparation of 50% Ethanol from 95% Ethanol</a> appeared first on <a href="https://www.laboratorynotes.com">Laboratory Notes</a>.</p>
]]></description>
		
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		<title>Preparation of 70% Ethanol from 95% Ethanol</title>
		<link>https://www.laboratorynotes.com/preparation-of-70-ethanol-from-95-ethanol/</link>
					<comments>https://www.laboratorynotes.com/preparation-of-70-ethanol-from-95-ethanol/#respond</comments>
		
		<dc:creator><![CDATA[admin]]></dc:creator>
		<pubDate>Thu, 12 Jan 2023 08:02:41 +0000</pubDate>
				<category><![CDATA[Reagents]]></category>
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		<guid isPermaLink="false">https://www.laboratorynotes.com/?p=11061</guid>

					<description><![CDATA[<p>OVERVIEW70% Ethanol is used as a disinfectant in labs. It is also used for washing DNA pellet to remove excess...</p>
<p>The post <a href="https://www.laboratorynotes.com/preparation-of-70-ethanol-from-95-ethanol/">Preparation of 70% Ethanol from 95% Ethanol</a> appeared first on <a href="https://www.laboratorynotes.com">Laboratory Notes</a>.</p>
]]></description>
		
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		<title>Preparation of 1M Sodium Phosphate Buffer, pH 7.2</title>
		<link>https://www.laboratorynotes.com/preparation-of-1m-sodium-phosphate-buffer-ph-7-2/</link>
					<comments>https://www.laboratorynotes.com/preparation-of-1m-sodium-phosphate-buffer-ph-7-2/#respond</comments>
		
		<dc:creator><![CDATA[admin]]></dc:creator>
		<pubDate>Sun, 08 Jan 2023 10:05:46 +0000</pubDate>
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		<guid isPermaLink="false">https://www.laboratorynotes.com/?p=10969</guid>

					<description><![CDATA[<p>OVERVIEWSodium Phosphate Buffer is a widely used biological buffer (buffer range: 5.8 &#8211; 8.0). Usually, It is prepared by mixing...</p>
<p>The post <a href="https://www.laboratorynotes.com/preparation-of-1m-sodium-phosphate-buffer-ph-7-2/">Preparation of 1M Sodium Phosphate Buffer, pH 7.2</a> appeared first on <a href="https://www.laboratorynotes.com">Laboratory Notes</a>.</p>
]]></description>
		
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		<title>Preparation of 0.1M Sodium Phosphate Buffer of Desired pH</title>
		<link>https://www.laboratorynotes.com/preparation-of-0-1m-sodium-phosphate-buffer-of-desired-ph/</link>
					<comments>https://www.laboratorynotes.com/preparation-of-0-1m-sodium-phosphate-buffer-of-desired-ph/#respond</comments>
		
		<dc:creator><![CDATA[admin]]></dc:creator>
		<pubDate>Sun, 08 Jan 2023 06:58:56 +0000</pubDate>
				<category><![CDATA[Reagents]]></category>
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		<guid isPermaLink="false">https://www.laboratorynotes.com/?p=10967</guid>

					<description><![CDATA[<p>Sodium Phosphate Buffer has a buffering range between 5.8 &#8211; 8.0. By mixing monobasic (NaH2PO4) and dibasic (Na2HPO4) sodium phosphate...</p>
<p>The post <a href="https://www.laboratorynotes.com/preparation-of-0-1m-sodium-phosphate-buffer-of-desired-ph/">Preparation of 0.1M Sodium Phosphate Buffer of Desired pH</a> appeared first on <a href="https://www.laboratorynotes.com">Laboratory Notes</a>.</p>
]]></description>
		
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		<title>Preparation of 1M Sodium Phosphate Monobasic (NaH2PO4)</title>
		<link>https://www.laboratorynotes.com/preparation-of-1m-sodium-phosphate-monobasic-nah2po4/</link>
					<comments>https://www.laboratorynotes.com/preparation-of-1m-sodium-phosphate-monobasic-nah2po4/#comments</comments>
		
		<dc:creator><![CDATA[admin]]></dc:creator>
		<pubDate>Fri, 06 Jan 2023 14:20:00 +0000</pubDate>
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		<guid isPermaLink="false">https://www.laboratorynotes.com/?p=10949</guid>

					<description><![CDATA[<p>OVERVIEWA 1M Sodium phosphate monobasic (also called Monosodium phosphate and Sodium dihydrogen phosphate) solution can be prepared by dissolving 119.98...</p>
<p>The post <a href="https://www.laboratorynotes.com/preparation-of-1m-sodium-phosphate-monobasic-nah2po4/">Preparation of 1M Sodium Phosphate Monobasic (NaH&lt;strong&gt;&lt;sub&gt;2&lt;/sub&gt;PO&lt;sub&gt;4&lt;/sub&gt;)&lt;/strong&gt;</a> appeared first on <a href="https://www.laboratorynotes.com">Laboratory Notes</a>.</p>
]]></description>
		
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		<title>Preparation of 3 M Sodium Acetate (CH3COONa) Solution, pH 5.2</title>
		<link>https://www.laboratorynotes.com/preparation-of-3-m-sodium-acetate-ch3coona-solution-ph-5-2/</link>
					<comments>https://www.laboratorynotes.com/preparation-of-3-m-sodium-acetate-ch3coona-solution-ph-5-2/#respond</comments>
		
		<dc:creator><![CDATA[admin]]></dc:creator>
		<pubDate>Sat, 31 Dec 2022 11:15:50 +0000</pubDate>
				<category><![CDATA[Reagents]]></category>
		<category><![CDATA[Recipes]]></category>
		<category><![CDATA[Solution]]></category>
		<category><![CDATA[ch3coona]]></category>
		<category><![CDATA[sodium acetate]]></category>
		<guid isPermaLink="false">https://www.laboratorynotes.com/?p=10816</guid>

					<description><![CDATA[<p>Sodium acetate is a sodium salt of acetic acid which dissolves readily in water. It is commercially supplied as both anhydrous (molecular weight: 82.03) and trihydrate (molecular weight: 136.08) forms and both can be used to prepare 3M Sodium acetate solution. Since anhydrous sodium acetate absorbs water from the atmosphere, weighing it accurately is difficult as exposure to air can cause anhydrous sodium acetate to turn to hydrated form.</p>
<p>Here we show a procedure to prepare 100 ml of 3M Sodium acetate, pH 5.2 from sodium acetate anhydrous. Briefly, to prepare a 100 ml solution of 3M Sodium acetate of pH 5.2, dissolve 24.61 g Sodium acetate anhydrous in 80 ml water, adjust the pH 5.2 using acetic acid, and then make the final volume to 100 ml. Remember that this solution act as a buffer solution (Acetic acid-Sodium acetate buffer)</p>
<p>The post <a href="https://www.laboratorynotes.com/preparation-of-3-m-sodium-acetate-ch3coona-solution-ph-5-2/">Preparation of 3 M Sodium Acetate (CH3COONa) Solution, pH 5.2</a> appeared first on <a href="https://www.laboratorynotes.com">Laboratory Notes</a>.</p>
]]></description>
		
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		<title>Preparation of Cell Lysis Buffer for Co-IP Containing Tris, NaCl, EDTA, NP-40, and Glycerol</title>
		<link>https://www.laboratorynotes.com/preparation-of-cell-lysis-buffer-for-co-ip-containing-tris-nacl-edta-np-40-and-glycerol/</link>
					<comments>https://www.laboratorynotes.com/preparation-of-cell-lysis-buffer-for-co-ip-containing-tris-nacl-edta-np-40-and-glycerol/#respond</comments>
		
		<dc:creator><![CDATA[admin]]></dc:creator>
		<pubDate>Sun, 18 Dec 2022 10:57:56 +0000</pubDate>
				<category><![CDATA[Reagents]]></category>
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		<guid isPermaLink="false">https://www.laboratorynotes.com/?p=10721</guid>

					<description><![CDATA[<p>OVERVIEWCo-immunoprecipitation requires intact interaction among proteins in order to co-immunoprecipitate protein complexes with the target protein. To keep the interactions...</p>
<p>The post <a href="https://www.laboratorynotes.com/preparation-of-cell-lysis-buffer-for-co-ip-containing-tris-nacl-edta-np-40-and-glycerol/">Preparation of Cell Lysis Buffer for Co-IP Containing Tris, NaCl, EDTA, NP-40, and Glycerol</a> appeared first on <a href="https://www.laboratorynotes.com">Laboratory Notes</a>.</p>
]]></description>
		
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		<title>Preparation of 10% (w/v) Sodium Hydroxide (NaOH) Solution</title>
		<link>https://www.laboratorynotes.com/preparation-of-10-w-v-sodium-hydroxide-naoh-solution/</link>
					<comments>https://www.laboratorynotes.com/preparation-of-10-w-v-sodium-hydroxide-naoh-solution/#comments</comments>
		
		<dc:creator><![CDATA[admin]]></dc:creator>
		<pubDate>Sat, 17 Dec 2022 14:57:26 +0000</pubDate>
				<category><![CDATA[Reagents]]></category>
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		<guid isPermaLink="false">https://www.laboratorynotes.com/?p=10698</guid>

					<description><![CDATA[<p>Here we have described a procedure to prepare 10 % sodium hydroxide (NaOH) solution. A 10% NaOH solution can be prepared by dissolving 10 g of NaOH in water to a final volume of 100 ml. Since dissolution of NaOH in water is a highly exothermic reaction, extreme care must be taken while preparing the solution.</p>
<p>The post <a href="https://www.laboratorynotes.com/preparation-of-10-w-v-sodium-hydroxide-naoh-solution/">Preparation of 10% (w/v) Sodium Hydroxide (NaOH) Solution</a> appeared first on <a href="https://www.laboratorynotes.com">Laboratory Notes</a>.</p>
]]></description>
		
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		<title>Preparation of 1N Sodium Hydroxide (NaOH) Solution by Diluting 10N Solution</title>
		<link>https://www.laboratorynotes.com/preparation-of-1n-sodium-hydroxide-naoh-solution-by-diluting-10n-solution/</link>
					<comments>https://www.laboratorynotes.com/preparation-of-1n-sodium-hydroxide-naoh-solution-by-diluting-10n-solution/#comments</comments>
		
		<dc:creator><![CDATA[admin]]></dc:creator>
		<pubDate>Sat, 17 Dec 2022 11:53:15 +0000</pubDate>
				<category><![CDATA[Reagents]]></category>
		<category><![CDATA[Recipes]]></category>
		<category><![CDATA[Solution]]></category>
		<category><![CDATA[naoh]]></category>
		<category><![CDATA[sodium hydroxide]]></category>
		<guid isPermaLink="false">https://www.laboratorynotes.com/?p=10692</guid>

					<description><![CDATA[<p>OVERVIEW A 1N solution of Sodium hydroxide (NaOH) solution can easily be prepared by diluting if you have already concentrated...</p>
<p>The post <a href="https://www.laboratorynotes.com/preparation-of-1n-sodium-hydroxide-naoh-solution-by-diluting-10n-solution/">Preparation of 1N Sodium Hydroxide (NaOH) Solution by Diluting 10N Solution</a> appeared first on <a href="https://www.laboratorynotes.com">Laboratory Notes</a>.</p>
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		<title>Preparation of 10N Sodium Hydroxide (NaOH)</title>
		<link>https://www.laboratorynotes.com/preparation-of-10n-sodium-hydroxide-naoh/</link>
					<comments>https://www.laboratorynotes.com/preparation-of-10n-sodium-hydroxide-naoh/#comments</comments>
		
		<dc:creator><![CDATA[admin]]></dc:creator>
		<pubDate>Sat, 17 Dec 2022 09:46:34 +0000</pubDate>
				<category><![CDATA[Reagents]]></category>
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		<guid isPermaLink="false">https://www.laboratorynotes.com/?p=10689</guid>

					<description><![CDATA[<p>The normality and molarity of the Sodium hydroxide (NaOH) solution are the same. This means the 10N solution of sodium...</p>
<p>The post <a href="https://www.laboratorynotes.com/preparation-of-10n-sodium-hydroxide-naoh/">Preparation of 10N Sodium Hydroxide (NaOH)</a> appeared first on <a href="https://www.laboratorynotes.com">Laboratory Notes</a>.</p>
]]></description>
		
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		<title>Preparation of 4% Paraformaldehyde Solution in PBS</title>
		<link>https://www.laboratorynotes.com/preparation-of-4-paraformaldehyde-solution-in-pbs/</link>
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		<dc:creator><![CDATA[admin]]></dc:creator>
		<pubDate>Sun, 27 Dec 2020 11:45:40 +0000</pubDate>
				<category><![CDATA[Reagents]]></category>
		<category><![CDATA[Recipes]]></category>
		<category><![CDATA[Solution]]></category>
		<category><![CDATA[formaldehyde]]></category>
		<category><![CDATA[paraformaldehyde]]></category>
		<guid isPermaLink="false">http://www.laboratorynotes.com/?p=1797</guid>

					<description><![CDATA[<p>A 100 ml of 4% (w/v) paraformaldehyde can be prepared by dissolving 4g of paraformaldehyde in PBS to a final volume of 100 ml.</p>
<p>The post <a href="https://www.laboratorynotes.com/preparation-of-4-paraformaldehyde-solution-in-pbs/">Preparation of 4% Paraformaldehyde Solution in PBS</a> appeared first on <a href="https://www.laboratorynotes.com">Laboratory Notes</a>.</p>
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