A 1M Sodium phosphate monobasic (also called Monosodium phosphate and Sodium dihydrogen phosphate) solution can be prepared by dissolving 119.98 g of Sodium phosphate monobasic (Na2HPO4, Molecular weight 119.98) in deionized water to a final volume of 1000 ml.
Reagents and solutions
Sodium phosphate monobasic, anhydrous (Na2HPO4) (Molecular weight: 119.98)
Deionized / Distilled water
Equipment and disposables
Conical flask / Beaker
1 M Sodium phosphate monobasic
Preparation of 1000 ml of 1M Sodium phosphate monobasic solution in water from Sodium phosphate monobasic (Na2HPO4)
Use personal protective equipment (lab coat, gloves, goggles, etc) for your safety and follow the guidelines of your institute.
Step 1: To prepare 1000 ml of 1M Sodium phosphate monobasic solution, weigh out 119.98 g of Sodium phosphate monobasic (Molecular weight : 119.98). Transfer to a 2 L beaker/conical flask. Add 800 ml of deionized/distilled water. Mix until sodium phosphate monobasic dissolves completely.
Anhydrous Sodium phosphate monobasic is readily converted into hydrated forms due to its hygroscopic nature.
One can use manual shaking using a glass pipette to mix the ingredients. Magnetic stirrer makes the dissolving process easy and convenient.
Do not dissolve in 1000 ml of deionized / Distilled water. In most cases, solution volume increases when a large amount of solute dissolves in the solvent.
Step 2: Adjust the volume to 1000 ml with deionized / Distilled water. Mix it again.
The solution will appear colorless and transparent.
Step 4: Transfer the solution to an autoclavable bottle. Sterilize the solution by autoclaving (20 minutes at 15 lb/sq.in. (psi) from 121-124°C on liquid cycle).
Solution can be stored at room temperature.
- Preparation of Sodium phosphate buffer
|Follow the table to prepare Sodium phosphate monobasic (Na2HPO4) solution of different concentrations and volumes.|
|Conc. / Volume||10 ml||50 ml||100 ml||250 ml||500 ml||1000 ml|
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