A 10% (weight/volume) sodium hydroxide (NaOH) stock solution can be prepared by dissolving 10 g of NaOH pellet in deionized/distilled water to a final volume of 100 ml. The detailed procedure is described below.
Reagents and solutions
Sodium hydroxide (NaOH) pallet
Deionized/Double distilled water
Equipment and disposables
Measuring cylinder/volumetric flask
Erlenmeyer flask / Beaker
Glass rod/Magnetic stirrer
Ice bucket with ice (optional)
10% NaOH solution
Preparation of 100 ml of 10% (w/v) NaOH solution in water
Use personal protective equipment (lab coat, gloves, goggles, etc) for your safety and follow the guidelines of your institute.
Step 1: To prepare 100 ml of 10% NaOH solution, weigh out 10 g of NaOH (molecular weight: 40).
Don’t expose solid NaOH to air for a long time while weighing. NaOH is hygroscopic in nature and absorbs water from the atmosphere quickly.
Step 2: Dissolve NaOH pellets in 70 ml water by adding small quantities of NaOH in water.
◊ Take 70 ml of deionized/double distilled water in a beaker.
◊ Add a few pellets (2 – 2 pellets each time) of NaOH in water and wait until they dissolve completely. Repeat this until all 10 g NaOH pellets are dissolved in water.
1. The dissolution of NaOH is a highly exothermic reaction, which can even break the glass beakers. It is recommended to keep the beaker on ice while dissolving NaOH.
2. Never add 70 ml water to 10 g NaOH pellet.
Step 3: Adjust the solution volume to 100 ml with deionized/double distilled water.
◊ Transfer solution to a measuring cylinder/volumetric flask (capacity: 100 ml) and adjust the volume to 100 ml with deionized/double distilled water.
The solution can be stored at room temperature for several months.
There is no need to sterilize this solution by autoclaving. No microorganism will grow in a 10% (w/v) NaOH solution.
|Follow the table to prepare NaOH solution of specific concentration and volume|
|Conc. / Volume||50 ml||100 ml||250 ml||500 ml||1000 ml|
|1%||0.5 g||1 g||2.5 g||5 g||10 g|
|2%||1 g||2 g||5 g||10 g||20 g|
|5%||2.5 g||5 g||12.5 g||25 g||50 g|
|10%||5 g||10 g||25 g||50 g||100 g|
Use calculator to calculate the quantities of NaOH for the preparation of NaOH solution of specific concentration and volume
Molecular weight of NaOH: 40
Volume of Sodium hydroxide solution: ml
(change the volume of the solution)
Molarity of Sodium hydroxide solution: %
(Change the molarity of the solution)
Amount of sodium hydroxide: 10 g