OVERVIEW
A 1L aqueous solution of 50% Ethanol can be prepared by diluting 95% ethanol. To prepare 1L of 50% ethanol, take 526.32 ml of 95% ethanol and make up the final volume to 1L with water.
REQUIREMENTS
Reagents and solutions
95% Ethanol
Deionized water
Equipment and disposables
Measuring cylinder
COMPOSITION
50% aqueous solution of ethanol
OBJECTIVE
Preparation of 1L of 50% ethanol in water
PREPARATION:
Use personal protective equipment (lab coat, gloves, goggles, etc) for your safety and follow your institute’s guidelines.
Step 1: Take 526.32 ml of 95% ethanol in a measuring cylinder.
Step 2: Adjust the final volume to 1000 ml with deionized water. Mix it.
Note: You need to add 473.68 ml water.
STORAGE
Solution can be stored at room temperature.
| Follow the table to prepare 50% Ethanol of different volumes. | ||||
| Ingredients / Volume | 100 ml | 250 ml | 500 ml | 1000 ml |
| 95% Ethanol | 52.63 ml | 131.58 ml | 263.16 ml | 526.32 ml |
Water | 47.37 ml | 118.42 ml | 236.84 ml | 473.68 ml |
