A 1L aqueous solution of 50% Ethanol can be prepared by diluting 95% ethanol. To prepare 1L of 50% ethanol, take 526.32 ml of 95% ethanol and make up the final volume to 1L with water.
Reagents and solutions
Equipment and disposables
50% aqueous solution of ethanol
Preparation of 1L of 50% ethanol in water
Use personal protective equipment (lab coat, gloves, goggles, etc) for your safety and follow your institute’s guidelines.
Step 1: Take 526.32 ml of 95% ethanol in a measuring cylinder.
Step 2: Adjust the final volume to 1000 ml with deionized water. Mix it.
Note: You need to add 473.68 ml water.
Solution can be stored at room temperature.
|Follow the table to prepare 50% Ethanol of different volumes.|
|Ingredients / Volume|