Preparation of 50% Ethanol from 95% Ethanol

A 1L aqueous solution of 50% Ethanol can be prepared by diluting 95% ethanol. To prepare 1L of 50% ethanol, take 526.32 ml of 95% ethanol and make up the final volume to 1L with water.


Reagents and solutions
95% Ethanol
Deionized water

Equipment and disposables
Measuring cylinder

50% aqueous solution of ethanol

Preparation of 1L of 50% ethanol in water


Use personal protective equipment (lab coat, gloves, goggles, etc) for your safety and follow your institute’s guidelines.

Step 1: Take 526.32 ml of 95% ethanol in a measuring cylinder.

Step 2: Adjust the final volume to 1000 ml with deionized water. Mix it.

Note: You need to add 473.68 ml water.

Solution can be stored at room temperature.

Follow the table to prepare 50% Ethanol of different volumes.
Ingredients / Volume

100 ml

250 ml

500 ml

1000 ml

95% Ethanol

52.63 ml

131.58 ml

263.16 ml

526.32 ml


47.37 ml

118.42 ml

236.84 ml

473.68 ml

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