Stock solution Archives - Laboratory Notes

Preparation of Poly-D-Lysine Hydrobromide Stock Solution (1 mg/ml)

admin — Sat, 21 Jan 2023 13:29:22 +0000

Poly-d-lysine promotes the adhesion and good growth of mammalian cells to the glass/plastic dishes. Here we describe a procedure to prepare a 1 mg/ml stock solution of Poly-d-lysine. The easiest way to prepare the solution is to buy a 5 mg poly-d-lysine (pack-size) and dissolve all of them in autoclaved sterile miliQ water without weighing it out. This stock solution can be used for coating glass coverslips to allow cells to grow adherently.

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Preparation of 16% Paraformaldehyde Stock Solution

admin — Sat, 14 Jan 2023 12:24:42 +0000

OVERVIEW A stock solution of 16% paraformaldehyde can be prepared by dissolving 16 g of paraformaldehyde in water to a...

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Preparation of 1M Sodium Phosphate Dibasic (Na2HPO4)

admin — Thu, 05 Jan 2023 12:41:39 +0000

A 1M Sodium phosphate dibasic solution can be prepared by dissolving 141.96 g of Sodium phosphate dibasic (Na2HPO4, Molecular weight 141.96) in deionized water in a total volume of 1000 ml.

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Preparation of 10x Tris-Glycine-SDS Running Buffer for SDS-PAGE using 20% SDS Solution

admin — Fri, 09 Dec 2022 14:47:27 +0000

OVERVIEW Tris-Glycine-SDS running buffer is commonly used to resolve proteins in SDS-polyacrylamide gel. A concentrated buffer is usually prepared that...

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Preparation of Stock Solution of Sodium Orthovanadate (0.2 M Na3VO4 in Water)

admin — Tue, 15 Nov 2022 22:17:45 +0000

OVERVIEW A 0.2 M (or 200 mM) stock solution (100x concentrated) of Sodium orthovanadate (Na3VO4) can be prepared by dissolving...

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Preparation of 1N Hydrochloric Acid

admin — Sat, 17 Sep 2022 08:28:18 +0000

Hydrochloric acid is supplied as a concentrated stock solution [37% (w/w)]. The molarity of the 37% (w/w) solution is 12.178...

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Preparation of Stock Solution of G-418 (100 mg/ml in water)

admin — Mon, 05 Sep 2022 18:52:31 +0000

OVERVIEW G-418 (also called G418, or G 418) is a selective antibiotic that is effective against many live organisms including...

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Preparation of 50 mg/ml Gentamicin Stock Solution

admin — Mon, 25 Apr 2022 03:57:04 +0000

OVERVIEW Gentamicin is an aminoglycoside antibiotic. It is effective against a wide variety of aerobic bacteria including E. coli. A...

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Preparation of 20% Tween 20 Solution

admin — Fri, 15 Apr 2022 08:51:44 +0000

OVERVIEW Tween 20 is a non-ionic detergent and is used in many biological applications including immunochemistry and western blotting. Tween...

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Preparation of 20% Triton X-100 Solution

admin — Wed, 23 Mar 2022 05:33:15 +0000

OVERVIEW Triton X-100 is a non-ionic, mild, non-denaturing detergent, used for solubilizing cell membrane, without denaturing proteins. It is often...

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Preparation 10% Sodium Azide Stock Solution

admin — Sat, 19 Mar 2022 04:47:57 +0000

OVERVIEW Commercially, sodium azide is supplied as a white powder. It is added to a solution to prevent the growth...

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Preparation of 10 M Sodium Hydroxide from 50% (w/w) Stock Solution

admin — Sun, 24 Oct 2021 07:29:05 +0000

OVERVIEW A 50% (w/w) Sodium Hydroxide can be purchased from several commercial suppliers. It is a clear colorless liquid and...

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Preparation of 1M MOPS (Free Acid) Solution

admin — Thu, 09 Sep 2021 18:27:35 +0000

Overview MOPS (Free acid, C7H15NO4S, Molecular weight: 209.26) is a zwitterionic buffer, useful for several cell and molecular biology applications...

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Preparation of 6X DNA Loading Buffer (Orange G, Xylene Cyanol FF and Ficoll 400)

admin — Tue, 07 Sep 2021 14:13:53 +0000

A 10 ml of 6X DNA loading dye can be prepared by dissolving and mixing 40 mg orange G, 25 mg xylene cyanol FF, 1.5 g Ficoll 400 and 0.1 ml of 1M Tris.Cl, pH 7.6 in sterile deionized or distilled water to a final volume of 10 ml.

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Preparation of SOB (Super Optimal Broth) Medium

admin — Mon, 19 Jul 2021 05:35:39 +0000

SOB (Super Optimal Broth) medium is a nutrient-rich microbial growth medium, designed by Douglas Hanahan in 1983. SOB or its glucose-supplemented version SOC is often used during transformation of E. coli competent cells with plasmids (added in transformed cells after heat shock). It results in better survival and higher transformation efficiency as compared to regular LB medium. It contains 2% Bacto-tryptone, 0.5% Bacto-yeast extract, 0.05% Sodium chloride, 2.5 mM KCl, and 10 mM MgCl2. A 1000 ml SOB medium is prepared by dissolving 20 g bacto-tryptone, 5 g bacto-yeast extract, 0.5 g sodium chloride and 2.5 ml of 1M KCl in water to a final volume of 1000 ml. Medium is sterilized by autoclaving. MgCl2 (5 ml of 2M MgCl2) is added just before use.

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Preparation of Alkaline Phosphatase Buffer

admin — Sun, 18 Jul 2021 12:44:43 +0000

Overview: An alkaline phosphatase buffer is required for the detection of alkaline phosphatase activity. It contains 5 – 50 mM...

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Preparation of 1M IPTG Solution

admin — Sat, 27 Mar 2021 22:15:16 +0000

Overview: In molecular biology laboratories, IPTG is commonly used to induce the expression of recombinant proteins in E. coli and...

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Preparation of Tris-saturated Phenol

admin — Fri, 01 Jan 2021 22:56:26 +0000

Overview: Phenol is a colorless crystalline solid. Phenol gradually turns pink to brownish color due to oxidation upon exposure to...

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Preparation of 10 M Sodium Hydroxide (NaOH) Solution

admin — Wed, 11 Nov 2020 01:20:25 +0000

Here we have described a procedure to prepare 10 M (or 10 N) sodium hydroxide (NaOH) solution. A 10 M NaOH solution can be prepared by dissolving 40 g of NaOH (Molecular Weight: 40.00) in water to a final volume of 100 ml. Since dissolution of NaOH in water is a highly exothermic reaction, extreme care must be taken while preparing the solution.

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Preparation of 1 M Tris.Cl Buffer

admin — Sun, 13 Sep 2020 07:50:13 +0000

Overview Tris is also known as THAM, Tris base, Trizma, Tris(hydroxymethyl)aminomethane. Tris is one of the most frequently used buffers...

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