Tween 20 is a non-ionic detergent and is used in many biological applications including immunochemistry and western blotting. Tween 20 is a highly viscous liquid. Accurately transferring a right volume of Tween 20 is difficult due to its viscous nature, therefore, a 20% Tween 20 solution can be prepared. In contrast to Triton X-100, Tween 20 is easily miscible in water.
Reagents and solution
20% Tween 20 in water
Preparation of 50 ml of 20% Tween 20 in water
Use personal protective equipment (lab coat, gloves, goggles, etc) for your safety and follow the guidelines of your institute.
Step 1: Weigh out 10.95 g of Tween 20 in a 50 ml Screw cap tube.
The density of Tween 20 is 1.095 g/mL at 25 °C. This means that 1 ml of Tween 20 weighs 1.095 grams and 10 ml will weigh 10.95 grams.
Step 2: Add 40 ml water and mix by inverting the tube several times/using a Tube Rotator until you see a clear transparent homogeneous liquid.
Unlike Triton X-100, Tween 20 dissolves readily in water.
A 20% Tween 20 solution can be stored at room temperature. Cover the tube with aluminum foil to protect tween-20 from light.