OVERVIEW

Commercially, sodium azide is supplied as a white powder. It is added to a solution to prevent the growth of microorganisms. It can also inhibit horseradish peroxidase enzyme activity. 

REQUIREMENTS

Reagents and solution
Sodium azide (e.g., Sigma-Aldrich, # S2002)
Deionized/Milli Q water

Equipment and disposables
15-ml disposable graduated tube (e.g., SARSTEDT, 62.554.502)
Electronic balance
Tube rotator (optional)

COMPOSITION

10% sodium azide in water

Objective

Preparation of 10% sodium azide solution in water

PROCEDURE

Use appropriate personal protective equipment (lab coat, gloves, goggles, etc) for your safety and follow the guidelines of your institute.

Precaution: Sodium azide is toxic. It should be handled carefully.

Step 1: Weight out 1 g of sodium azide in a 15-ml disposable graduated tube.

Step 2: Add 8 ml water and mix by inverting the tube a few times or use a tube rotator.

Note:
Sodium azide readily dissolves in water. 

Step 3: Adjust the final volume 10 ml with water.

Storage

Store the solution at room temperature.