Commercially, sodium azide is supplied as a white powder. It is added to a solution to prevent the growth of microorganisms. It can also inhibit horseradish peroxidase enzyme activity.
Reagents and solution
Sodium azide (e.g., Sigma-Aldrich, # S2002)
Deionized/Milli Q water
Equipment and disposables
15-ml disposable graduated tube (e.g., SARSTEDT, 62.554.502)
Tube rotator (optional)
10% sodium azide in water
Preparation of 10% sodium azide solution in water
Use appropriate personal protective equipment (lab coat, gloves, goggles, etc) for your safety and follow the guidelines of your institute.
Precaution: Sodium azide is toxic. It should be handled carefully.
Step 1: Weight out 1 g of sodium azide in a 15-ml disposable graduated tube.
Step 2: Add 8 ml water and mix by inverting the tube a few times or use a tube rotator.
Sodium azide readily dissolves in water.
Step 3: Adjust the final volume 10 ml with water.
Store the solution at room temperature.