Preparation of 1M Sodium Phosphate Dibasic (Na2HPO4)

A 1M Sodium phosphate dibasic (also called Disodium phosphate and disodium hydrogen phosphate) solution can be prepared by dissolving 141.96 g of Sodium phosphate dibasic (Na2HPO4, Molecular weight 141.96) in deionized water to a final volume of 1000 ml.


Reagents and solutions
Sodium phosphate dibasic, anhydrous (Na2HPO4) (Molecular weight: 141.96)
Deionized / Distilled water

Equipment and disposables
Measuring cylinder
Conical flask / Beaker
Magnetic stirrer

1 M Sodium phosphate dibasic

Preparation of 1000 ml of 1M Sodium phosphate dibasic solution in water from Sodium phosphate dibasic  (Na2HPO4)


Use personal protective equipment (lab coat, gloves, goggles, etc) for your safety and follow the guidelines of your institute.

Step 1: To prepare 1000 ml of 1M Sodium phosphate dibasic solution, weigh out 141.96 g of  Sodium phosphate dibasic (Molecular weight = 141.96). Transfer to a 2 L  beaker/conical flask. Add 800 ml of deionized/distilled water. Mix until sodium phosphate dibasic dissolves completely.

Anhydrous Sodium phosphate dibasic is readily converted into hydrated forms due to its hygroscopic nature.

One can use manual shaking using a glass pipette to mix the ingredients. Magnetic stirrer makes the dissolving process easy and convenient.

Do not dissolve in 1000 ml of deionized / Distilled water. In most cases, solution volume increases when a large amount of solute dissolves in the solvent.

Step 2: Adjust the volume to 1000 ml with deionized / Distilled water. Mix it again.

The solution will appear colorless and transparent.

Step 4: Transfer the solution to an autoclavable bottle. Sterilize the solution by autoclaving (20 minutes at 15 lb/ (psi) from 121-124°C on liquid cycle).

Solution can be stored at room temperature.


  • Preparation of Sodium phosphate buffer
Follow the table to prepare Sodium phosphate dibasic (Na2HPO4) solution of different concentrations and volumes.
Conc. / Volume10 ml50 ml100 ml250 ml500 ml1000 ml

0.1 M

0.14 g

0.8 g

1.42 g

3.55 g

7.1 g

14.2 g

0.5 M

0.71 g

3.55 g

7.1 g

17.745 g

35.49 g

70.98 g

1 M

1.42 g

7.98 g

14.196 g

35.49 g

70.98 g

141.96 g

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