Reagents Archives - Laboratory Notes

Water Freezing: Understanding the Process

admin — Tue, 04 Feb 2025 09:17:25 +0000

Further reading: Bernal, J. D., & Fowler, R. H., 1933. A theory of water and ionic solution, with particular reference...

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Preparation of Potassium Chloride-based 10x PCR Buffer

admin — Fri, 24 Mar 2023 05:10:32 +0000

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Preparation of 0.1 mg/ml Poly-D-Lysine Solution from Stock Solution

admin — Sat, 21 Jan 2023 18:59:55 +0000

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Preparation of Poly-D-Lysine Hydrobromide Stock Solution (1 mg/ml)

admin — Sat, 21 Jan 2023 13:29:22 +0000

Poly-d-lysine promotes the adhesion and good growth of mammalian cells to the glass/plastic dishes. Here we describe a procedure to prepare a 1 mg/ml stock solution of Poly-d-lysine. The easiest way to prepare the solution is to buy a 5 mg poly-d-lysine (pack-size) and dissolve all of them in autoclaved sterile miliQ water without weighing it out. This stock solution can be used for coating glass coverslips to allow cells to grow adherently.

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Preparation of 4% Paraformaldehyde Solution in PBS from 16% Stock Solution

admin — Sat, 14 Jan 2023 14:19:30 +0000

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Preparation of 16% Paraformaldehyde Stock Solution

admin — Sat, 14 Jan 2023 12:24:42 +0000

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Preparation of 50% Ethanol from 95% Ethanol

admin — Thu, 12 Jan 2023 10:03:28 +0000

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Preparation of 70% Ethanol from 95% Ethanol

admin — Thu, 12 Jan 2023 08:02:41 +0000

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Preparation of 1M Sodium Phosphate Buffer, pH 7.2

admin — Sun, 08 Jan 2023 10:05:46 +0000

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Preparation of 0.1M Sodium Phosphate Buffer of Desired pH

admin — Sun, 08 Jan 2023 06:58:56 +0000

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Preparation of 1M Sodium Phosphate Monobasic (NaH2PO4)

admin — Fri, 06 Jan 2023 14:20:00 +0000

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Preparation of 1M Sodium Phosphate Dibasic (Na2HPO4)

admin — Thu, 05 Jan 2023 12:41:39 +0000

A 1M Sodium phosphate dibasic solution can be prepared by dissolving 141.96 g of Sodium phosphate dibasic (Na2HPO4, Molecular weight 141.96) in deionized water in a total volume of 1000 ml.

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Preparation of 3 M Sodium Acetate (CH3COONa) Solution, pH 5.2

admin — Sat, 31 Dec 2022 11:15:50 +0000

Sodium acetate is a sodium salt of acetic acid which dissolves readily in water. It is commercially supplied as both anhydrous (molecular weight: 82.03) and trihydrate (molecular weight: 136.08) forms and both can be used to prepare 3M Sodium acetate solution. Since anhydrous sodium acetate absorbs water from the atmosphere, weighing it accurately is difficult as exposure to air can cause anhydrous sodium acetate to turn to hydrated form.

Here we show a procedure to prepare 100 ml of 3M Sodium acetate, pH 5.2 from sodium acetate anhydrous. Briefly, to prepare a 100 ml solution of 3M Sodium acetate of pH 5.2, dissolve 24.61 g Sodium acetate anhydrous in 80 ml water, adjust the pH 5.2 using acetic acid, and then make the final volume to 100 ml. Remember that this solution act as a buffer solution (Acetic acid-Sodium acetate buffer)

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Preparation of Cell Lysis Buffer for Co-IP Containing Tris, NaCl, EDTA, NP-40, and Glycerol

admin — Sun, 18 Dec 2022 10:57:56 +0000

The post Preparation of Cell Lysis Buffer for Co-IP Containing Tris, NaCl, EDTA, NP-40, and Glycerol appeared first on Laboratory Notes.

Preparation of 10% (w/v) Sodium Hydroxide (NaOH) Solution

admin — Sat, 17 Dec 2022 14:57:26 +0000

Here we have described a procedure to prepare 10 % sodium hydroxide (NaOH) solution. A 10% NaOH solution can be prepared by dissolving 10 g of NaOH in water to a final volume of 100 ml. Since dissolution of NaOH in water is a highly exothermic reaction, extreme care must be taken while preparing the solution.

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Preparation of 1N Sodium Hydroxide (NaOH) Solution by Diluting 10N Solution

admin — Sat, 17 Dec 2022 11:53:15 +0000

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Preparation of 10N Sodium Hydroxide (NaOH)

admin — Sat, 17 Dec 2022 09:46:34 +0000

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Preparation of 10x Tris-Glycine-SDS Running Buffer for SDS-PAGE using 20% SDS Solution

admin — Fri, 09 Dec 2022 14:47:27 +0000

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Preparation of 0.5 M Sodium Fluoride Solution

admin — Thu, 17 Nov 2022 18:53:43 +0000

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Preparation of Stock Solution of Sodium Orthovanadate (0.2 M Na3VO4 in Water)

admin — Tue, 15 Nov 2022 22:17:45 +0000

The post Preparation of Stock Solution of Sodium Orthovanadate (0.2 M Na3VO4 in Water) appeared first on Laboratory Notes.