- Synonyms:
- Culture Type: Cell line
- Keywords: Human epithelial-like lung adenocarcinoma (NSCLC) cell line
- Description: The cell line, HCC2935, was established from the pleural effusion of a White, 39-year-old, male patient with adenocarcinoma.
- Source:
- Cell line features:
- Morphology: Epithelial-like
- Cell Type: Epithelial cell
- Growth mode: Adherent
- Life span: Infinite (continuous culture)
- Karyotype:
- Doubling time:
- ≈105 h (ATCC)
- 70.4 hours (cellosaurus)
- Sequence variations (gene mutations/deletions/fusions/translocations):
- EGFR (E746 – T751 deletion, S752I mutation in Tyrosine kinase domain of EGFR)
- PTEN (L108R mutation) – Likely Gain-of-function (depmap)
- KLF5 (D418H mutation) – Likely Gain-of-function (depmap)
- TP53 (Y220C mutation) – Loss-of-function (depmap)
- For all gene mutations, please visit https://depmap.org/portal/cell_line/HCC2935_LUNG?tab=mutations
- Gene expression:
- –
- Morphology: Epithelial-like
- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: RPMI-1640 + 10% FBS
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO2, 95% Air
- Temperature: Humidified 37°C incubator
- Subculturing:
- Subculturing method: Enzymatic dissociation
- Cell dissociation solution: 0.25% (w/v) Trypsin- 0.53 mM EDTA solution
- Harvesting time: Sub-confluent cultures (≈80% confluent, Density: 1 x 105 cells/cm2 )
- Split ratio: 1:4 to 1:6
- Seeding densities: 5 x 103 to 7 x 103 viable cells/cm2
- Medium Renewal: Every 2 to 3 days
- Cryopreservation:
- Freeze medium: Complete growth medium + 5% DMSO
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density): 2 – 4 x 106 viable cells/ml
- Volume per vial: 1 ml
- Freeze medium: Complete growth medium + 5% DMSO
- Applications:
- Cancer research
- 3D cell culture
- Availability
- American Type Culture Collection (ATCC): Product link
- References
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
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