Category: Lab Notes: Biotechniques
Trehalose as Cryoprotectant
Trehalose is a non‑permeating cryoprotectant that stabilizes membranes, protects proteins, and reduces ice formation. This article explains its mechanisms and applications in cryobiology.
Glass Transition Temperature
The glass transition temperature (Tg) marks the point where biological solutions become glass‑like and molecular motion stops. This article explains Tg, its mechanism, and its importance in cryopreservation.
ITS Liquid Media Supplement
ITS Liquid Media Supplement provides insulin, transferrin, and selenium to support serum‑free cell culture, metabolic stability, and high‑viability growth. This article explains its composition, biological function, and major suppliers.
Sterile Liquid Handling: Pipetting and Dispensing Using a Micropipette
Sterile liquid handling ensures accurate, contamination‑free transfer of samples in microbiology, cell culture, and pharmaceutical work. This article explains essential pipetting and dispensing techniques used to maintain sterility and precision in laboratory environments.
TensorFlow
TensorFlow is an open-source software library developed by the Google Brain Team for numerical computation and large-scale machine learning. It…
Keras
Keras is an open-source, high-level neural network library designed to simplify the creation, training, and deployment of deep learning models….
PyTorch
PyTorch is an open-source deep learning framework developed by Facebook’s AI Research Lab (FAIR) and officially released in 2016. It…
DNA Microarray
A DNA microarray is a powerful molecular biology tool used to measure the expression levels of thousands of genes simultaneously…
Mechanism of Chain Termination in Sanger Sequencing
In Sanger sequencing, a mixture of normal deoxyribonucleotides (dNTPs) and modified dideoxyribonucleotides (ddNTPs) is used to control DNA chain elongation. …
Sanger Sequencing: Historical Foundations to Modern Refinements
Sanger Sequencing, also known as the chain-termination method, stands as one of the most significant breakthroughs in molecular biology. Developed…
Sanger Sequencing Vs Next-Generation Sequencing
Criteria Sanger Sequencing Next-Generation Sequencing (NGS) Remarks Definition A first-generation DNA sequencing method based on selective incorporation of chain-terminating dideoxynucleotides…
High-Throughput Genomic Sequencing Technology
High-throughput genomic sequencing technologies, often referred to as next-generation sequencing (NGS), represent a transformative advancement in molecular biology and medicine,…
Sequencing
Sequencing refers to the experimental process of determining the precise order of the building blocks that make up a biological…
Common Substrates of Horseradish Peroxidase
Horseradish peroxidase (HRP) is widely used in immunoassays and histological techniques because of its ability to catalyze the oxidation of…
Immunohistochemistry
Immunohistochemistry (IHC) is a widely used laboratory technique that combines histology, immunology, and chemistry to detect and visualize the presence…