Category: Lab Notes: Biotechniques
Simoa (Single Molecule Array)
The Simoa (Single Molecule Array) technique is a highly sensitive digital immunoassay technology that allows for the detection of extremely…
TOPtkLuciferase Assay for Wnt Signaling
The TOPtkLuciferase reporter assay is a specific type of luciferase reporter system designed to monitor activity of the Wnt/β-catenin signaling…
Luciferase Assay
The luciferase assay is a powerful and widely used technique in molecular and cellular biology for monitoring gene expression, promoter…
Biolayer Interferometry
Biolayer Interferometry (BLI) is a label-free, optical biosensing technique used to monitor biomolecular interactions in real time. It is widely…
Quartz Crystal Microbalance (QCM) Immunosensors
Quartz Crystal Microbalance (QCM) immunosensors are highly sensitive biosensors that combine the principles of immunoassays with acoustic sensing technology to…
Mass Spectrometry Immunoassays
Mass spectrometry immunoassays (MSIAs) are powerful analytical techniques that combine the high specificity of immunoaffinity capture with the precision and…
Electrochemical Immunoassay
Electrochemical immunoassays are a class of biosensing techniques that combine the specificity of immunoassays with the sensitivity and simplicity of…
Immunoelectrophoresis
Immunoelectrophoresis is a laboratory technique that combines the principles of electrophoresis and immunodiffusion to separate and identify proteins, particularly antigens,…
Gene Expression Analysis
Gene expression analysis refers to the examination and quantification of the activity of genes in a particular cell or tissue…
nCounter® Analysis System (NanoString Technologies)
The nCounter® Analysis System, developed by NanoString Technologies, is a robust and highly multiplexed platform designed for the direct, digital…
Gene Expression Analysis by NanoString Technology
NanoString technology is a highly sensitive and multiplexed platform for the direct quantification of nucleic acids, particularly messenger RNA (mRNA),…
GeneChip™ Human Exon 1.0 ST Array
The GeneChip™ Human Exon 1.0 ST Array, developed by Affymetrix (now part of Thermo Fisher Scientific), is a high-resolution microarray…
Microarray
Microarrays are powerful analytical tools used to simultaneously measure the expression levels, presence, or interactions of thousands of biological molecules—most…
Surface Plasmon Resonance
Surface Plasmon Resonance (SPR) is a sophisticated and widely adopted analytical technique used to study biomolecular interactions in real time…
Radioimmunoassay
Radioimmunoassay (RIA) is a highly sensitive and specific technique used to measure minute concentrations of antigens—such as hormones, drugs, or…