Category: Stock solution

Poly-d-lysine promotes the adhesion and good growth of mammalian cells to the glass/plastic dishes. Here we describe a procedure to prepare a 1 mg/ml stock solution of Poly-d-lysine. The easiest way to prepare the solution is to buy a 5 mg poly-d-lysine (pack-size) and dissolve all of them in autoclaved sterile miliQ water without weighing it out. This stock solution can be used for coating glass coverslips to allow cells to grow adherently.

OVERVIEW A stock solution of 16% paraformaldehyde can be prepared by dissolving 16 g of paraformaldehyde in water to a…

A 1M Sodium phosphate dibasic solution can be prepared by dissolving 141.96 g of Sodium phosphate dibasic (Na2HPO4, Molecular weight 141.96) in deionized water in a total volume of 1000 ml.

OVERVIEW Tris-Glycine-SDS running buffer is commonly used to resolve proteins in SDS-polyacrylamide gel. A concentrated buffer is usually prepared that…

OVERVIEW A 0.2 M (or 200 mM) stock solution (100x concentrated) of Sodium orthovanadate (Na3VO4) can be prepared by dissolving…

Hydrochloric acid is supplied as a concentrated stock solution [37% (w/w)]. The molarity of the 37% (w/w)  solution is 12.178…

OVERVIEW G-418 (also called G418, or G 418) is a selective antibiotic that is effective against many live organisms including…

OVERVIEW Gentamicin is an aminoglycoside antibiotic. It is effective against a wide variety of aerobic bacteria including E. coli. A…

OVERVIEW Tween 20 is a non-ionic detergent and is used in many biological applications including immunochemistry and western blotting. Tween…

OVERVIEW Triton X-100 is a non-ionic, mild, non-denaturing detergent, used for solubilizing cell membrane, without denaturing proteins. It is often…

OVERVIEW Commercially, sodium azide is supplied as a white powder. It is added to a solution to prevent the growth…

OVERVIEW A 50% (w/w) Sodium Hydroxide can be purchased from several commercial suppliers. It is a clear colorless liquid and…

Overview MOPS (Free acid, C7H15NO4S, Molecular weight: 209.26) is a zwitterionic buffer, useful for several cell and molecular biology applications…

A 10 ml of 6X DNA loading dye can be prepared by dissolving and mixing 40 mg orange G, 25 mg xylene cyanol FF, 1.5 g Ficoll 400 and 0.1 ml of 1M Tris.Cl, pH 7.6 in sterile deionized or distilled water to a final volume of 10 ml.

SOB (Super Optimal Broth) medium is a nutrient-rich microbial growth medium, designed by Douglas Hanahan in 1983. SOB or its glucose-supplemented version SOC is often used during transformation of E. coli competent cells with plasmids (added in transformed cells after heat shock). It results in better survival and higher transformation efficiency as compared to regular LB medium. It contains 2% Bacto-tryptone, 0.5% Bacto-yeast extract, 0.05% Sodium chloride, 2.5 mM KCl, and 10 mM MgCl2. A 1000 ml SOB medium is prepared by dissolving 20 g bacto-tryptone, 5 g bacto-yeast extract, 0.5 g sodium chloride and 2.5 ml of 1M KCl in water to a final volume of 1000 ml. Medium is sterilized by autoclaving. MgCl2 (5 ml of 2M MgCl2) is added just before use.