Category: Stock solution
Preparation of Poly-D-Lysine Hydrobromide Stock Solution (1 mg/ml)
Poly-d-lysine promotes the adhesion and good growth of mammalian cells to the glass/plastic dishes. Here we describe a procedure to prepare a 1 mg/ml stock solution of Poly-d-lysine. The easiest way to prepare the solution is to buy a 5 mg poly-d-lysine (pack-size) and dissolve all of them in autoclaved sterile miliQ water without weighing it out. This stock solution can be used for coating glass coverslips to allow cells to grow adherently.
Preparation of 16% Paraformaldehyde Stock Solution
OVERVIEW A stock solution of 16% paraformaldehyde can be prepared by dissolving 16 g of paraformaldehyde in water to a…
Preparation of 1M Sodium Phosphate Dibasic (Na2HPO4)
A 1M Sodium phosphate dibasic solution can be prepared by dissolving 141.96 g of Sodium phosphate dibasic (Na2HPO4, Molecular weight 141.96) in deionized water in a total volume of 1000 ml.
Preparation of 10x Tris-Glycine-SDS Running Buffer for SDS-PAGE using 20% SDS Solution
OVERVIEW Tris-Glycine-SDS running buffer is commonly used to resolve proteins in SDS-polyacrylamide gel. A concentrated buffer is usually prepared that…
Preparation of Stock Solution of Sodium Orthovanadate (0.2 M Na3VO4 in Water)
OVERVIEW A 0.2 M (or 200 mM) stock solution (100x concentrated) of Sodium orthovanadate (Na3VO4) can be prepared by dissolving…
Preparation of 1N Hydrochloric Acid
Hydrochloric acid is supplied as a concentrated stock solution [37% (w/w)]. The molarity of the 37% (w/w) solution is 12.178…
Preparation of Stock Solution of G-418 (100 mg/ml in water)
OVERVIEW G-418 (also called G418, or G 418) is a selective antibiotic that is effective against many live organisms including…
Preparation of 50 mg/ml Gentamicin Stock Solution
OVERVIEW Gentamicin is an aminoglycoside antibiotic. It is effective against a wide variety of aerobic bacteria including E. coli. A…
Preparation of 20% Tween 20 Solution
OVERVIEW Tween 20 is a non-ionic detergent and is used in many biological applications including immunochemistry and western blotting. Tween…
Preparation of 20% Triton X-100 Solution
OVERVIEW Triton X-100 is a non-ionic, mild, non-denaturing detergent, used for solubilizing cell membrane, without denaturing proteins. It is often…
Preparation 10% Sodium Azide Stock Solution
OVERVIEW Commercially, sodium azide is supplied as a white powder. It is added to a solution to prevent the growth…
Preparation of 10 M Sodium Hydroxide from 50% (w/w) Stock Solution
OVERVIEW A 50% (w/w) Sodium Hydroxide can be purchased from several commercial suppliers. It is a clear colorless liquid and…
Preparation of 1M MOPS (Free Acid) Solution
Overview MOPS (Free acid, C7H15NO4S, Molecular weight: 209.26) is a zwitterionic buffer, useful for several cell and molecular biology applications…
Preparation of 6X DNA Loading Buffer (Orange G, Xylene Cyanol FF and Ficoll 400)
A 10 ml of 6X DNA loading dye can be prepared by dissolving and mixing 40 mg orange G, 25 mg xylene cyanol FF, 1.5 g Ficoll 400 and 0.1 ml of 1M Tris.Cl, pH 7.6 in sterile deionized or distilled water to a final volume of 10 ml.
Preparation of SOB (Super Optimal Broth) Medium
SOB (Super Optimal Broth) medium is a nutrient-rich microbial growth medium, designed by Douglas Hanahan in 1983. SOB or its glucose-supplemented version SOC is often used during transformation of E. coli competent cells with plasmids (added in transformed cells after heat shock). It results in better survival and higher transformation efficiency as compared to regular LB medium. It contains 2% Bacto-tryptone, 0.5% Bacto-yeast extract, 0.05% Sodium chloride, 2.5 mM KCl, and 10 mM MgCl2. A 1000 ml SOB medium is prepared by dissolving 20 g bacto-tryptone, 5 g bacto-yeast extract, 0.5 g sodium chloride and 2.5 ml of 1M KCl in water to a final volume of 1000 ml. Medium is sterilized by autoclaving. MgCl2 (5 ml of 2M MgCl2) is added just before use.