Category: Reagents

Preparation of 5M Hydrochloric Acid

OVERVIEW 5M hydrochloric acid can be prepared by diluting a concentrated 37% (w/w) stock solution of hydrochloric acid. A 37%…

Preparation of 1N Hydrochloric Acid

Hydrochloric acid is supplied as a concentrated stock solution [37% (w/w)]. The molarity of the 37% (w/w)  solution is 12.178…

Preparation of Stock Solution of G-418 (100 mg/ml in water)

OVERVIEW G-418 (also called G418, or G 418) is a selective antibiotic that is effective against many live organisms including…

Preparation of 50 mg/ml Gentamicin Stock Solution

OVERVIEW Gentamicin is an aminoglycoside antibiotic. It is effective against a wide variety of aerobic bacteria including E. coli. A…

Preparation of 20% Tween 20 Solution

OVERVIEW Tween 20 is a non-ionic detergent and is used in many biological applications including immunochemistry and western blotting. Tween…

Preparation of 20% Triton X-100 Solution

OVERVIEW Triton X-100 is a non-ionic, mild, non-denaturing detergent, used for solubilizing cell membrane, without denaturing proteins. It is often…

Preparation 10% Sodium Azide Stock Solution

OVERVIEW Commercially, sodium azide is supplied as a white powder. It is added to a solution to prevent the growth…

Preparation of 10 M Sodium Hydroxide from 50% (w/w) Stock Solution

OVERVIEW A 50% (w/w) Sodium Hydroxide can be purchased from several commercial suppliers. It is a clear colorless liquid and…

Preparation of 1M MOPS (Free Acid) Solution

Overview MOPS (Free acid, C7H15NO4S, Molecular weight: 209.26) is a zwitterionic buffer, useful for several cell and molecular biology applications…

Preparation of 6X DNA Loading Buffer (Orange G, Xylene Cyanol FF and Ficoll 400)

A 10 ml of 6X DNA loading dye can be prepared by dissolving and mixing 40 mg orange G, 25 mg xylene cyanol FF, 1.5 g Ficoll 400 and 0.1 ml of 1M Tris.Cl, pH 7.6 in sterile deionized or distilled water to a final volume of 10 ml.

Sodium Hydroxide (NaOH)

Synonyms Caustic soda, Sodium Hydroxide, NaOH Appearance Colourless/white crystalline solid Odor Odorless Molecular weight 39.9971 Molar mass 39.9971 g/mol Melting…

Preparation of SOB (Super Optimal Broth) Medium

SOB (Super Optimal Broth) medium is a nutrient-rich microbial growth medium, designed by Douglas Hanahan in 1983. SOB or its glucose-supplemented version SOC is often used during transformation of E. coli competent cells with plasmids (added in transformed cells after heat shock). It results in better survival and higher transformation efficiency as compared to regular LB medium. It contains 2% Bacto-tryptone, 0.5% Bacto-yeast extract, 0.05% Sodium chloride, 2.5 mM KCl, and 10 mM MgCl2. A 1000 ml SOB medium is prepared by dissolving 20 g bacto-tryptone, 5 g bacto-yeast extract, 0.5 g sodium chloride and 2.5 ml of 1M KCl in water to a final volume of 1000 ml. Medium is sterilized by autoclaving. MgCl2 (5 ml of 2M MgCl2) is added just before use.

Preparation of Alkaline Phosphatase Buffer

Overview: An alkaline phosphatase buffer is required for the detection of alkaline phosphatase activity.  It contains 5 – 50 mM…

Suppliers – Ampicillin Sodium salt

Ampicillin Sodium salt can be purchased from any of the following suppliers. You must visit the supplier’s homepage to order…

Ampicillin Sodium Salt

Synonym D-(−)-α-Aminobenzylpenicillin sodium saltSodium ampicillinAmpicillin sodiumAmpicillin sodium salt CAS Number 69-52-3 Beilstein Registry Number 4119211 EC Number 200-708-1 MDL number…