Preparation of Potassium Chloride-based 10x PCR Buffer
Preparation of 0.1 mg/ml Poly-D-Lysine Solution from Stock Solution
Preparation of Poly-D-Lysine Hydrobromide Stock Solution (1 mg/ml)
Poly-d-lysine promotes the adhesion and good growth of mammalian cells to the glass/plastic dishes. Here we describe a procedure to prepare a 1 mg/ml stock solution of Poly-d-lysine. The easiest way to prepare the solution is to buy a 5 mg poly-d-lysine (pack-size) and dissolve all of them in autoclaved sterile miliQ water without weighing it out. This stock solution can be used for coating glass coverslips to allow cells to grow adherently.
Preparation of 4% Paraformaldehyde Solution in PBS from 16% Stock Solution
Preparation of 16% Paraformaldehyde Stock Solution
Preparation of 50% Ethanol from 95% Ethanol
Preparation of 70% Ethanol from 95% Ethanol
Preparation of 1M Sodium Phosphate Buffer, pH 7.2
Preparation of 0.1M Sodium Phosphate Buffer of Desired pH
Preparation of 1M Sodium Phosphate Monobasic (NaH2PO4)
Preparation of 1M Sodium Phosphate Dibasic (Na2HPO4)
Preparation of 3 M Sodium Acetate (CH3COONa) Solution, pH 5.2
Sodium acetate is a sodium salt of acetic acid which dissolves readily in water. It is commercially supplied as both anhydrous (molecular weight: 82.03) and trihydrate (molecular weight: 136.08) forms and both can be used to prepare 3M Sodium acetate solution. Since anhydrous sodium acetate absorbs water from the atmosphere, weighing it accurately is difficult as exposure to air can cause anhydrous sodium acetate to turn to hydrated form.
Here we show a procedure to prepare 100 ml of 3M Sodium acetate, pH 5.2 from sodium acetate anhydrous. Briefly, to prepare a 100 ml solution of 3M Sodium acetate of pH 5.2, dissolve 24.61 g Sodium acetate anhydrous in 80 ml water, adjust the pH 5.2 using acetic acid, and then make the final volume to 100 ml. Remember that this solution act as a buffer solution (Acetic acid-Sodium acetate buffer)
Preparation of Cell Lysis Buffer for Co-IP Containing Tris, NaCl, EDTA, NP-40, and Glycerol
Preparation of 10% (w/v) Sodium Hydroxide (NaOH) Solution
Here we have described a procedure to prepare 10 % sodium hydroxide (NaOH) solution. A 10% NaOH solution can be prepared by dissolving 10 g of NaOH in water to a final volume of 100 ml. Since dissolution of NaOH in water is a highly exothermic reaction, extreme care must be taken while preparing the solution.