Category: Lab Notes: Protein Science
Protein Folding
Protein folding is the complex process by which a linear chain of amino acids organizes itself into a specific three-dimensional…
Biuret Assay
The Biuret Assay, also known as Biuret Method, is a classical biochemical technique used for the quantification of proteins based…
Tryptophan
Tryptophan is a unique and essential aromatic amino acid that plays critical roles in numerous biological processes. With the molecular…
Tyrosine
Tyrosine is an aromatic amino acid that plays fundamental roles in protein structure and serves as a precursor for several…
Non-Destructive Protein Quantification Techniques
Non-destructive protein quantification techniques enable researchers to measure protein concentrations without consuming or altering the sample significantly, thus preserving it…
Colorimetric Assays for Protein Quantification
Colorimetric assays for protein quantification are fundamental biochemical techniques that rely on specific chemical reactions producing colored products proportional to…
Bradford Assay for Protein Quantification
Bradford Assay for protein quantification is a widely used colorimetric method based on the binding of Coomassie Brilliant Blue G-250…
Protein Quantification by UV Absorbance at 280 nm
Protein quantification by UV absorbance at 280 nm is a direct, non-destructive method based on the intrinsic absorption properties of…
Protein Quantification by Spectrophotometric Assays
Spectrophotometric measurement of protein concentration is a fundamental technique in biochemistry and molecular biology that enables researchers to quantify protein…
Protein Quantification
Protein quantification is an essential analytical process in molecular biology, biochemistry, and biotechnology, aimed at determining the concentration of proteins…
Protein Blotting
Protein blotting refers to transferring and immobilizing the proteins on a solid membrane support. The immobilized proteins, which are accessible…