Category: Lab Notes: Recombinant DNA Technology
Factors Influencing Plasmid Host-Range Specificity in Bacteria
The host-range specificity of a plasmid refers to the spectrum of bacterial species in which the plasmid can successfully replicate…
Plasmid DNA Vs Chromosomal DNA
Criteria Plasmid DNA Chromosomal DNA Remarks Definition Small, circular, double-stranded extrachromosomal DNA molecule found mainly in bacteria and some eukaryotes…
Semi-Quantitative Polymerase Chain Reaction (sqPCR)
Semi-Quantitative Polymerase Chain Reaction (sqPCR) is a conventional PCR-based technique used to estimate the relative abundance of nucleic acids in…
SYBR Green-based Quantitative PCR (qPCR)
SYBR Green-based Quantitative PCR (qPCR) is a widely utilized method for the detection and quantification of nucleic acids in real-time…
Sequence-specific Probe-based Quantitative PCR (qPCR)
Sequence-specific probe-based quantitative PCR (qPCR) is an advanced real-time PCR technique that enables highly specific and sensitive quantification of nucleic…
SYBR Green-based qPCR Vs Sequence-specific Probe-based qPCR
Criteria SYBR Green qPCR Sequence-Specific Probe-Based qPCR Remarks Detection Chemistry Non-specific DNA-binding dye (SYBR Green I) binds to any double-stranded…
Semi-quantitative PCR (sqPCR) Vs Quantitative PCR (qPCR)
Criteria Semi-Quantitative PCR (sqPCR) Quantitative PCR (qPCR) Remarks Purpose Estimates relative abundance of target nucleic acids by comparing band intensities…
Quantitative Polymerase Chain Reaction (qPCR)
Quantitative Polymerase Chain Reaction (qPCR), also known as real-time PCR, is an advanced molecular biology technique designed for the quantification…
Preparing Culture of Escherichia coli for Plasmid Isolation
Preparing a culture of plasmid-harboring Escherichia coli is the starting point in all plasmid isolation protocols. Suboptimal culture conditions and…
Preparation of Culture of Escherichia coli for Plasmid Minipreparation
A small-scale plasmid isolation procedure, the miniprep, yields a sufficient amount of plasmid for the screening of clones by restriction…
Restriction enzyme-free Cloning
In contrast to conventional cloning strategies, Restriction enzyme-free cloning (also called Restriction-free cloning) involves no treatment of DNA fragments to…
T4 DNA Ligase
T4 DNA ligase is a highly efficient enzyme derived from the T4 bacteriophage that catalyzes the formation of phosphodiester bonds…
TA Cloning
TA cloning involves base pairing of adenine (A) and thymine (T) (complementary base pairs) that result in the joining of…
Molecular Cloning (DNA Cloning)
Molecular cloning or DNA cloning refers to a set of methods that involve in generating identical copies of a piece…
Pfu DNA Polymerase
Pfu DNA polymerase is a high-fidelity, thermostable DNA-dependent DNA polymerase originally isolated from the hyperthermophilic archaeon Pyrococcus furiosus (Lundberg et…