Category: Recipes

Preparation of 0.1 mg/ml Poly-D-Lysine Solution from Stock Solution

OVERVIEWA 0.1 mg/ml Poly-d-lysine solution is used to coat coverslips. Coated coverslips allow cells to adhere and grow. A 0.1…

Preparation of 4% Paraformaldehyde Solution in PBS from 16% Stock Solution

OVERVIEW A 4% Paraformaldehyde Solution can be prepared by diluting a concentrated 16% paraformaldehyde stock solution. To prepare 100 ml…

Preparation of 50% Ethanol from 95% Ethanol

OVERVIEWA 1L aqueous solution of 50% Ethanol can be prepared by diluting 95% ethanol. To prepare 1L of 50% ethanol,…

Preparation of 70% Ethanol from 95% Ethanol

OVERVIEW70% Ethanol is used as a disinfectant in labs. It is also used for washing DNA pellet to remove excess…

Preparation of 1M Sodium Phosphate Buffer, pH 7.2

OVERVIEWSodium Phosphate Buffer is a widely used biological buffer (buffer range: 5.8 – 8.0). Usually, It is prepared by mixing…

Preparation of 0.1M Sodium Phosphate Buffer of Desired pH

Sodium Phosphate Buffer has a buffering range between 5.8 – 8.0. By mixing monobasic (NaH2PO4) and dibasic (Na2HPO4) sodium phosphate…

Preparation of 1M Sodium Phosphate Monobasic (NaH2PO4)

OVERVIEWA 1M Sodium phosphate monobasic (also called Monosodium phosphate and Sodium dihydrogen phosphate) solution can be prepared by dissolving 119.98…

Preparation of 1M Sodium Phosphate Dibasic (Na2HPO4)

A 1M Sodium phosphate dibasic solution can be prepared by dissolving 141.96 g of Sodium phosphate dibasic (Na2HPO4, Molecular weight 141.96) in deionized water in a total volume of 1000 ml.

Preparation of 3 M Sodium Acetate (CH3COONa) Solution, pH 5.2

Sodium acetate is a sodium salt of acetic acid which dissolves readily in water. It is commercially supplied as both anhydrous (molecular weight: 82.03) and trihydrate (molecular weight: 136.08) forms and both can be used to prepare 3M Sodium acetate solution. Since anhydrous sodium acetate absorbs water from the atmosphere, weighing it accurately is difficult as exposure to air can cause anhydrous sodium acetate to turn to hydrated form.

Here we show a procedure to prepare 100 ml of 3M Sodium acetate, pH 5.2 from sodium acetate anhydrous. Briefly, to prepare a 100 ml solution of 3M Sodium acetate of pH 5.2, dissolve 24.61 g Sodium acetate anhydrous in 80 ml water, adjust the pH 5.2 using acetic acid, and then make the final volume to 100 ml. Remember that this solution act as a buffer solution (Acetic acid-Sodium acetate buffer)

Preparation of Cell Lysis Buffer for Co-IP Containing Tris, NaCl, EDTA, NP-40, and Glycerol

OVERVIEWCo-immunoprecipitation requires intact interaction among proteins in order to co-immunoprecipitate protein complexes with the target protein. To keep the interactions…

Preparation of 10% Sodium Hydroxide Solution

Preparation of 10% (w/v) Sodium Hydroxide (NaOH) Solution

Here we have described a procedure to prepare 10 % sodium hydroxide (NaOH) solution. A 10% NaOH solution can be prepared by dissolving 10 g of NaOH in water to a final volume of 100 ml. Since dissolution of NaOH in water is a highly exothermic reaction, extreme care must be taken while preparing the solution.

Preparation of 1N Sodium Hydroxide (NaOH) Solution by Diluting 10N Solution

OVERVIEW A 1N solution of Sodium hydroxide (NaOH) solution can easily be prepared by diluting if you have already concentrated…

Preparation of 10N Sodium Hydroxide (NaOH)

The normality and molarity of the Sodium hydroxide (NaOH) solution are the same. This means the 10N solution of sodium…

Preparation of 0.5 M Sodium Fluoride Solution

OVERVIEW A 0.5 M (or 500 mM) stock solution (250x concentrated) of Sodium fluoride (NaF) can be prepared by dissolving…

Preparation of 5M Hydrochloric Acid

OVERVIEW 5M hydrochloric acid can be prepared by diluting a concentrated 37% (w/w) stock solution of hydrochloric acid. A 37%…