Category: Recipes

Preparation of 5M Hydrochloric Acid

OVERVIEW 5M hydrochloric acid can be prepared by diluting a concentrated 37% (w/w) stock solution of hydrochloric acid. A 37%…

Preparation of 1N Hydrochloric Acid

Hydrochloric acid is supplied as a concentrated stock solution [37% (w/w)]. The molarity of the 37% (w/w)  solution is 12.178…

Preparation of 20% Triton X-100 Solution

OVERVIEW Triton X-100 is a non-ionic, mild, non-denaturing detergent, used for solubilizing cell membrane, without denaturing proteins. It is often…

Preparation of 6X DNA Loading Buffer (Orange G, Xylene Cyanol FF and Ficoll 400)

A 10 ml of 6X DNA loading dye can be prepared by dissolving and mixing 40 mg orange G, 25 mg xylene cyanol FF, 1.5 g Ficoll 400 and 0.1 ml of 1M Tris.Cl, pH 7.6 in sterile deionized or distilled water to a final volume of 10 ml.

Preparation of 50X TAE Electrophoresis Buffer

A 50x TAE buffer can be prepared by mixing and dissolving 242 g Tris base, 100 ml of 0.5 M EDTA and 57.1 ml glacial acetic acid in a deionized water to a final volume of 1000 ml. The pH of the final solution should be between 8.2 – 8.4.