Category: Lab Notes

Restriction Enzyme

Restriction enzymes belong to a group of enzymes called endonucleases. They are also called restriction endonucleases or restrictase. These enzymes…

AlwI Restriction Enzyme

Endonuclease Type IIS Restriction enzyme Cleaves double-stranded DNA outside of specific recognition site Recognizes asymmetric DNA sequences (see fig) DNA…

Millimolar – Micromolar Conversion

Formula: 1 Millimolar = 1000 Micromolar 0.001 Millimolar = 1 Micromolar More: 0.001 Millimolar = 1 Micromolar 0.01 Millimolar =…

How to Calculate Molarity of 37% (w/w) Hydrochloric acid (HCl), Alternative Method

OVERVIEW Hydrochloric acid is an aqueous solution of hydrogen chloride gas. The density of 37% (w/w) hydrochloric acid solution is…

Molar – Micromolar Conversion

Formula: 1 Molar = 1000,000  Micromolar 0.000001 Molar = 1 Micromolar More: 0.000001 Molar = 1 Micromolar 0.00001 Molar =…

Millimolar – Nanomolar Conversion

Formula: 1 Millimolar = 1000,000  Nanomolar 0.000001 Millimolar = 1 Nanomolar More 0.000001 Millimolar = 1  Nanomolar 0.00001 Millimolar =…

Deoxyribose

Molecular formula C5H10O4 Molecular weight 134.131 Molar mass  134.131g/mol IUPAC name  2-deoxy-d-ribose CAS Number  533-67-5 Chemically, Deoxyribose (or 2-deoxyribose) is…

Nitrogenous Bases

Nitrogenous bases (also known as Nucleobases or bases) are nitrogen-containing compounds. They are an important component of nucleic acids. These…

EcoRI Restriction Enzyme

Endonuclease Type II Restriction enzyme Cleaves double-stranded DNA at specific cleavage sites Recognize hexameric palindromic sequence Sticky end cutter (cuts…

PmeI Restriction Enzyme

Synonyms: MssI Endonuclease Type II Restriction enzyme Cleaves double-stranded DNA at specific sites Recognize octameric palindromic sequence Blunt-end cutter (cut…

NheI Restriction Enzyme

Endonuclease Type II Restriction enzyme Cleaves double-stranded DNA at specific sites Recognize hexameric palindromic sequence Sticky end cutter (cut both…

Ribose

Chemically, Ribose is a single-ring 5-carbon (pentose) sugar. Ribose is an essential component of ribonucleic acid (RNA). It can exist…

SmaI Restriction Enzyme

Endonuclease Type II Restriction enzyme Cleaves double-stranded DNA at specific recognition site Recognize hexameric palindromic sequence Blunt-end cutter (cut both…