Category: Lab Notes

1903: The chromosome theory of inheritance was developed independently by two researchers: Walter S. Sutton and Theodor Boveri 1910: The…

Properties Plasmid Virus Remarks Replication Autonomous, requires host enzyme Autonomous, requires host enzyme   Relationship with host More symbiotic than…

Properties Plasmid Episome Remarks Molecular nature DNA DNA Both of them are made up of deoxyribonucleic acid Genetic material type…

The Borrelia burgdorferi genome consists of a linear chromosome and at least 21 plasmids of which 12 are linear and…

Causes Lyme disease in humans Gram-negative bacteria Microaerophile Motile spirocheteGenome consists of a linear chromosome. It also contains at least…

AGS cell line grows adherently as a monolayer.  This cell line does not have a tendency to form true foci….

The 35-mm dishes are small rounded dishes. The diameter of these dishes is 35 mm. However, the diameter (excluding the…

Plasmid Plasmid pSRQ700 Plasmid Isolation Basic Steps in Plasmid Isolation Growing Escherichia coli for Plasmid Isolation Principle of Alkaline Lysis…

The term “Plasmid” was first used by Joshua Lederberg in 1952 (Lederberg, 1952). Plasmids are extrachromosomal, double-stranded, covalently closed circular…

pEC156 ColE1 pCoo REFERENCES Werbowy & Kaczorowski, 2016. Plasmid pEC156, a Naturally Occurring Escherichia coli Genetic Element That Carries Genes…

Resistance to antibiotics Production of antibiotics Degradation of complex organic compounds Production of bacteriocins Production of enterotoxins Restriction and modification…

cag pathogenicity island (cag PAI) CagA Flagella IceA LPS Lewisx,y antigens Neutrophil-activating protein (NAP) Outer inflammatory protein (OipA) PicB Peptidoglycan…

NOTES Helicobacter pylori Type IV Secretion System (T4SS) of Helicobacter pylori GENES CagA (Cytotoxin-Associated Gene A) CagA/E-cadherin interaction EXPERIMENTAL SUPPORT…

Helicobacter pylori was discovered by J R Warren and B Marshall. Their work was published in 1983 in a Journal “The Lancet”.

Different strains of Helicobacter pylori exhibit high genetic heterogeneity. REFERENCES Research Articles Alm et al., 2000. Comparative genomics of Helicobacter…