Category: Lab Notes

Ponceau S (Tetrasodium Salt)

Synonyms Ponceau S, Ponceau S sodium salt, Ponceau S tetrasodium, Acid Red 112 Formal Name 3-hydroxy-4-[2-[2-sulfo-4-[2-(4-sulfophenyl)diazenyl]phenyl]diazenyl]-2,7-naphthalenedisulfonic acid, tetrasodium salt Molecular…

Staining PVDF Membrane with Ponceau S after Protein Transfer

Overview: Like nitrocellulose membrane, PVDF membrane can also be stained with Ponceau S stain to reveal protein bands after western…

Ponceau S

Ponceau S belongs to a group of azo dyes that can stain proteins in red/pink color. Chemically it is a…

Ponceau S Staining of Nitrocellulose Membrane after Western Blotting

Overview: Staining of nitrocellulose membrane using Ponceau S is often used to evaluate the quality and transfer efficiency of western…

Preparation of Ponceau S Staining Solution

Overview: Ponceau S stain is often used to evaluate the transfer efficiency of western blotting. It reversibly stains the protein…

Cell/Tissue Culture Vessels: Flasks, Plates and Dishes

A variety of cell/tissue culture vessels are available commercially and can be purchased from various suppliers. These vessels are specifically…

Transfection

Transfection is a very common and powerful method to deliver nucleic acid (DNA/RNA) into tissue culture cells. Usually, the purpose…

Molarity of 32% (w/w) Hydrochloric Acid (HCl)

The molarity of 32% (w/w) Hydrochloric Acid is 10.181 M. OVERVIEW A 32% (w/w) Hydrochloric Acid is a clear colorless…

Topic: Hydrochloric acid (HCl)

NOTES Hydrogen Chloride (HCl) Hydrochloric Acid CALCULATIONS Hydrogen Chloride (HCl) Molecular Weight Calculation     Molarity of 37% (w/w) Hydrochloric Acid (HCl)…

Preparation of 1M Acetic Acid Solution from Glacial Acetic Acid

Overview: A 1M Acetic acid solution can be prepared by diluting the concentrated acetic acid stock solution. Glacial acetic acid,…

Topic: Acetic Acid

ChemDB: Acetic Acid Acetic Acid (CH3COOH) Molecular Weight Calculation Suppliers: Glacial Acetic Acid How to Calculate Molarity of Glacial Acetic…

H-1152

ROCK inhibitor

Y-27632

Cell-permeable Highly selective and potent inhibitor of Rho-associated protein kinases ROCK1 (Ki = 220 nM) and ROCK2 (Ki = 300…

List of Epithelial-specific Integrins

The following integrins are considered specific to epithelial cells. α1β1 α2β1  α3β1 α5β1 α6β1  α9β1  αvβ5  αvβ6  α6β4