Category: Lab Notes

Plasmid: Timeline of Key Events

1903: The chromosome theory of inheritance was developed independently by two researchers: Walter S. Sutton and Theodor Boveri 1910: The…

Plasmid vs Virus

Properties Plasmid Virus Remarks Replication Autonomous, requires host enzyme Autonomous, requires host enzyme   Relationship with host More symbiotic than…

Plasmid vs Episome

Properties Plasmid Episome Remarks Molecular nature DNA DNA Both of them are made up of deoxyribonucleic acid Genetic material type…

Linear Plasmid

The Borrelia burgdorferi genome consists of a linear chromosome and at least 21 plasmids of which 12 are linear and…

Borrelia burgdorferi

Causes Lyme disease in humans Gram-negative bacteria Microaerophile Motile spirocheteGenome consists of a linear chromosome. It also contains at least…

AGS Cell Line Characteristics

AGS cell line grows adherently as a monolayer.  This cell line does not have a tendency to form true foci….

35-mm Dishes for Cell Culture

The 35-mm dishes are small rounded dishes. The diameter of these dishes is 35 mm. However, the diameter (excluding the…

Topic: Plasmid

Plasmid Plasmid pSRQ700 Plasmid Isolation Basic Steps in Plasmid Isolation Growing Escherichia coli for Plasmid Isolation Principle of Alkaline Lysis…

Plasmid Overview

Plasmid

The term “Plasmid” was first used by Joshua Lederberg in 1952 (Lederberg, 1952). Plasmids are extrachromosomal, double-stranded, covalently closed circular…

List of Naturally Occurring Plasmids in  Escherichia coli

pEC156 ColE1 pCoo REFERENCES Werbowy & Kaczorowski, 2016. Plasmid pEC156, a Naturally Occurring Escherichia coli Genetic Element That Carries Genes…

Plasmid-Encoded Traits

Resistance to antibiotics Production of antibiotics Degradation of complex organic compounds Production of bacteriocins Production of enterotoxins Restriction and modification…

List of Helicobacter pylori Virulence Factors

cag pathogenicity island (cag PAI) CagA Flagella IceA LPS Lewisx,y antigens Neutrophil-activating protein (NAP) Outer inflammatory protein (OipA) PicB Peptidoglycan…

Topic: Helicobacter pylori

NOTES Helicobacter pylori Type IV Secretion System (T4SS) of Helicobacter pylori GENES CagA (Cytotoxin-Associated Gene A) CagA/E-cadherin interaction EXPERIMENTAL SUPPORT…

Discovery of Helicobacter pylori

Helicobacter pylori was discovered by J R Warren and B Marshall. Their work was published in 1983 in a Journal “The Lancet”.

Genetic Heterogeneity in Helicobacter pylori Strains

Different strains of Helicobacter pylori exhibit high genetic heterogeneity. REFERENCES Research Articles Alm et al., 2000. Comparative genomics of Helicobacter…