Category: Lab Notes

Importance of Subculturing in Cell Culture

Cell culture is not static. Cells acquire changes when maintained in culture for an extended period (Masters & Stacey, 2007)….

Papain Digestion of Antibody/Immunoglobulin

Partial digestion of antibodies (immunoglobulins) with the protease papain produces three fragments. Among these, two identical fragments are known as…

Antibody Structure

Antibodies belong to the immunoglobulin superfamily. A typical immunoglobulin, such as IgG, is a large protein made up of four…

Antibody

Antibodies are glycoproteins with a molecular weight of approximately 160 kDa. They are produced by immune cells and are vital…

Cell Line: NCI-H1882

Cell Line: NCI-H1882 Synonyms: NCI-H1882 NCIH1882 H1882 H-1882 Culture Type: Cell line Keywords: Human Small Cell Lung Cancer Cell Line…

Cell Line: NCI-H2009

Cell Line: NCI-H2009 Synonyms: NCI-H2009 NCIH2009 H2009 H-2009 Culture Type: Cell line Keywords: Human Lung Adenocarcinoma Cell Line Description: Established…

Effects of Mycoplasma on Cultured Cells

Mycoplasma can affect cell culture in a variety of ways. These are: Morphological changes Slow proliferation rate Reduced saturation density…

Cell

A cell is the basic structural and functional unit of life. All living organisms are composed of cells. Depending on…

Cell Line: NCI-H2110

Cell Line: NCI-H2110 Synonyms: NCI-H2110 NCIH2110 H2110 H-2110 Culture Type: Cell line Keywords: Human Non-small Cell Lung Cancer Cell line …

Cell Line: NCI-H295R

Cell Line: NCI-H295R Synonyms: NCI-H295R NCIH295R H295R H-295R Culture Type: Cell line Keywords: Human Adrenocortical Carcinoma Cell Line Description: Parent…

Cell Line: NCI-H211

Cell Line: NCI-H211 Synonyms: NCI-H211 NCIH211 H211 H-211 Culture Type: Cell line Keywords: Human Small Cell Lung Carcinoma Cell Line…

Cell Line: HCC827

HCC827 – A Human epithelial-like lung adenocarcinoma (NSCLC) cell line, established from the lung of a white, 39-year-old female patient with adenocarcinoma.

Cell Line: 293-PERV-PK-CIRCE

Synonyms: 293-PERV-PK-CIRCE Description: Parent cell line: 293 (also known as HEK-293) Infected with endogenous retrovirus of pigs, produced by the…

Genetically Modified Organism (GMO)

Any organism, including animals, plants, and microbes whose genome is modified by genetic engineering techniques, is called a genetically modified…

Cell Line: HEK293-A GFP-WIPI1

Description: HEK293-A cells (subclone of HEK-293) stably expressing GFP-tagged human WIPI1 Synonyms: HEK293-A GFP-WIPI1 Keywords: HEK293-A cells (a subclone of…