Category: Lab Notes: Molecular Cloning
Gibson Assembly
Gibson Assembly is an efficient molecular cloning method that allows seamless assembly of multiple DNA fragments in a single reaction….
Molecular Cloning
Molecular cloning is a fundamental technique in molecular biology that enables the creation of identical DNA copies and their propagation…
dCas9 (Endonuclease Dead Cas9)
dCas9 is a mutated version of Cas 9 that lacks endonuclease activity (Endonuclease dead Cas9). It is created by point…
Targeting RNA using CRISPR-Cas
The following CRISPR-Cas systems can be used to target and modify RNA in cells. The following Cas proteins can recognize…