Category: Solution

Further reading: Bernal, J. D., & Fowler, R. H., 1933. A theory of water and ionic solution, with particular reference…

Sodium acetate is a sodium salt of acetic acid which dissolves readily in water. It is commercially supplied as both anhydrous (molecular weight: 82.03) and trihydrate (molecular weight: 136.08) forms and both can be used to prepare 3M Sodium acetate solution. Since anhydrous sodium acetate absorbs water from the atmosphere, weighing it accurately is difficult as exposure to air can cause anhydrous sodium acetate to turn to hydrated form.

Here we show a procedure to prepare 100 ml of 3M Sodium acetate, pH 5.2 from sodium acetate anhydrous. Briefly, to prepare a 100 ml solution of 3M Sodium acetate of pH 5.2, dissolve 24.61 g Sodium acetate anhydrous in 80 ml water, adjust the pH 5.2 using acetic acid, and then make the final volume to 100 ml. Remember that this solution act as a buffer solution (Acetic acid-Sodium acetate buffer)

Here we have described a procedure to prepare 10 % sodium hydroxide (NaOH) solution. A 10% NaOH solution can be prepared by dissolving 10 g of NaOH in water to a final volume of 100 ml. Since dissolution of NaOH in water is a highly exothermic reaction, extreme care must be taken while preparing the solution.

A 100 ml of 4% (w/v) paraformaldehyde can be prepared by dissolving 4g of paraformaldehyde in PBS to a final volume of 100 ml.