- Cell Line: LS 180
- Synonyms:
- LS-180
- LS 180
- Culture Type: Cell line
- Keywords: Human Epithelial Colorectal Adenocarcinoma Cell Line
- Description: Established from the colon of a White, 58-year-old female patient with colorectal adenocarcinoma
- Origin:
- Cell line features:
- Cell Type: Epithelial
- Morphology:-
- Growth mode: Adherent
- Life span: Infinite (continuous culture)
- Karyotype: Modal number = 45, range = 42 to 47
- Doubling time:-
- Tumorigenicity: Yes, in nude mice
- Products: The cells secrete CEA and mucin
- Gene expression: Interleukin 10, Interleukin 6
- Sequence variations:
- KRAS (G12D mutation, Oncogenic, Gain-of-function)
- GNB1 (S316G mutation, Oncogenic Gain-of-function)
- PIK3CA (H1047R mutation, Oncogenic, Gain-of-function)
- CTNNB1 (S45F mutation, Likely Oncogenic, Gain-of-function)
- PTEN (I67K mutation, Likely Oncogenic, Unknown)
- For more information, please visit
- Cell Type: Epithelial
- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium:
- EMEM + 10% FBS (ATCC)
- EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS). (culturecollections)
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO2, 95% Air
- Temperature: Humidified 37°C incubator
- Subculturing:
- Subculturing method: Mechanical dissociation of cells by repeated pipetting and scraping gently
- Harvesting time: Sub-confluent cultures (70-80%)
- Split ratio: 1:2 to 1:5
- Seeding density: 2-5 x 104 cells/cm²
- Medium Renewal: Two to three times per week
- Subculturing method: Mechanical dissociation of cells by repeated pipetting and scraping gently
- Cryopreservation:
- Freezing medium: Complete growth medium + 5% DMSO
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial:-
- Freezing medium: Complete growth medium + 5% DMSO
- Applications:
- Cancer research
- Tumourigenicity studies
- 3D cell culture
- Availability
- American Type Culture Collection (ATCC): Product link
- Sigma Aldrich: Product link
- UK Health Security Agency Culture Collections: Product link
- AcceGen: Product link
- AddexBio Technologies: Product link
- References
- Images:-
- LS-180 cell image (Source: depmap)
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
- EMEM (Eagle’s Minimum Essential Medium)
- CEA (Carcinoembryonic antigen)
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