- Cell Line: NCI-H510A
- Synonyms:
- NCI-H510A
- NCIH510A
- NCI-H510
- H510A
- H-510A
- H510
- H-510
- Culture Type: Cell line
- Keywords: Human Small Cell Lung Carcinoma Cell Line
- Description: Established from adrenal metastasis of a 56-year-old, Caucasian, male with Small cell lung carcinoma
- Origin:
- Organism:
- Species: Human (Homo sapiens)
- Age: 56 years
- Ethnicity: Caucasian
- Gender: Male
- Tissue (origin): Lung
- Tissue (isolation): Adrenal gland (metastatic site)
- Disease:
- Small Cell Lung Carcinoma
- Primary/Metastasis: Metastatic
- Small Cell Lung Carcinoma
- Year of origin: 1982
- Organism:
- Cell line features:
- Cell Type: Epithelial
- Morphology:
- Growth mode: Mixed – adherent and suspension (aggregates)
- Life span: Infinite (continuous culture)
- Karyotype: Hypotriploid, modal number = 54, range = 46 to 57
- Doubling time:
- 1 week (DSMZ)
- 66 hours (Cellosaurus)
- 1 week (DSMZ)
- Microsatellite instability: Stable
- Special features:-
- Product:-
- Tumorigenicity:- Yes, cells produce tumors in nude mice
- Gene expression:
- The cells express elevated levels of four biochemical markers of SCLC: neuron-specific enolase, the brain isoenzyme of creatine kinase, L-DOPA decarboxylase, and bombesin-like immunoreactivity.
- Sequence variations:
- TP53 (R282G mutation, Likely Oncogenic, Likely Loss-of-function, Homozygous)
- For information, please visit
- TP53 (R282G mutation, Likely Oncogenic, Likely Loss-of-function, Homozygous)
- Cell Type: Epithelial
- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium:
- F-12K Medium + 10% FBS (ATCC)
- RPMI 1640 : Ham’s F12 (at 1:1) + 10% heat-inactivated FBS (DSMZ)
- RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS). (culturecollections)
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO2, 95% Air
- Temperature: Humidified 37°C incubator
- Subculturing:
- Subculturing method: Mechanical dissociation from substratum and collecting suspension cells by centrifugation
- Harvesting time:-
- Split ratio: 1:3 to 1:8
- Medium Renewal: 2 to 3 times per week
- Subculturing method: Mechanical dissociation from substratum and collecting suspension cells by centrifugation
- Cryopreservation:
- Applications:
- Cancer research
- 3D cell culture
- Availability
- American Type Culture Collection (ATCC): Product link
- Cytion: Product link
- BCRJ (Banco de Células do Rio de Janeiro): Product link
- References
- ATCC
- Cellosaurus
- DepMap
- Carney et al., 1985. Establishment and identification of small cell lung cancer cell lines having classic and variant features. Cancer Res. 45(6), 2913-23. PMID-2985257
- Images:
- NCI-H510A cell image link (Source: ATCC)
- NCI-H510A cell image link (Source: Culture Collections)
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
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