- Cell Line: NCI-H378
- Synonyms:
- NCI-H378
- NCIH378
- H378
- H-378
- Culture Type: Cell line
- Keywords: Human Small Cell Lung Carcinoma Cell line
- Description: Established from the pleural effusion of a 66-year-old Caucasian female with a Small cell lung carcinoma, Stage E
- Origin:
- Organism:
- Species: Human (Homo sapiens)
- Age: 66 years
- Ethnicity: Caucasian
- Gender: Female
- Tissue (origin): Lung
- Tissue (isolation): Pleural effusion
- Disease:
- Small cell lung carcinoma, Stage E
- Primary/Metastasis: Metastatic
- Year of origin:
- Organism:
- Cell line features:
- Cell Type:
- Morphology:
- Growth mode: Suspension, multicellular aggregates
- Life span: Infinite (continuous culture)
- Karyotype:-
Doubling time: - Microsatellite instability: Stable
- Special features:
- Product:-
- Tumorigenicity: Produce tumors in athymic nude mice
- Gene expression:-
- Oncogene expression
- Positive for C-kit
- Positive for L-myc
- SCLC marker expression
- Elevated levels of neuron-specific enolase, the brain isoenzyme of creatine kinase, L-dopa carboxylase, and bombesin-like immunoreactivity
- Oncogene expression
- Sequence variations:
- TP53 (Y163C mutation, Likely Oncogenic, Loss-of-function, Homozygous)
- KDR (A1065T mutation, Likely Oncogenic, Gain-of-function)
- Amplification of L-myc
- For information, please visit
- Cell Type:
- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: RPMI-1640 Medium + 10% FBS
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO2, 95% Air
- Temperature: Humidified 37°C incubator
- Subculturing:
- Subculturing method: Addition or replacement of medium
- Harvesting time:-
- Seeding density:-
- Split ratio:-
- Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
- Subculturing method: Addition or replacement of medium
- Cryopreservation:
- Freezing medium:
- Complete growth medium + 5% DMSO
- Complete growth medium + 5% DMSO
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial:
- Freezing medium:
- Applications:
- Cancer research
- 3D cell culture
- Availability:
- American Type Culture Collection (ATCC): Product link
- References
- Images:
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
- SCLC (Small cell lung carcinoma)
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