- Cell Line: NCI-H2452
- Synonyms:
- NCI-H2452
- NCIH2452
- H2452
- H-2452
- Culture Type: Cell line
- Keywords: Human Lung Mesothelioma Cell line
- Description: Established from the lungs of a Caucasian male patient with mesothelioma
- Origin:
- Organism:
- Species: Human (Homo sapiens)
- Age:-
- Ethnicity: Caucasian
- Gender: Male
- Tissue (origin): Lung
- Tissue (isolation): Lung, pleura
- Disease:
- Mesothelioma
- Primary/Metastasis: Metastatic
- Year of origin: 1990
- Organism:
- Cell line features:
- Cell Type:
- Morphology: Epithelial
- Growth mode: Adherent
- Life span: Infinite (continuous culture)
- Karyotype:-
Doubling time: 48 hours - Microsatellite instability: Stable
- Special features:
- Product:-
- Soft agar colony formation assay:-
- Tumorigenicity:-
- Gene expression:
- Sequence variations:
- MPL (A490G mutation, Likely Oncogenic, Likely Gain-of-function)
- BAP1 mutation, Likely Oncogenic, Loss-of-function, Heterozygous)
- For information, please visit
- Cell Type:
- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: RPMI-1640 Medium + 10% FBS
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO2, 95% Air
- Temperature: Humidified 37°C incubator
- Subculturing:
- Subculturing method: Enzymatic dissociation
- Cell dissociation solution: 0.25% (w/v) Trypsin-0.53 mM EDTA solution
- Harvesting time:-
- Seeding density:-
- Split ratio: 1:4 to 1:6
- Medium Renewal: Every 2 to 3 days
- Cryopreservation:
- Freezing medium:
- Complete growth medium + 5% DMSO
- Complete growth medium + 5% DMSO
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial:
- Freezing medium:
- Applications:
- Cancer research
- 3D cell culture
- Availability:
- American Type Culture Collection (ATCC): Product link
- Cytion: Product link
- References
- Images:
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
- Article Authenticity Index *
- Please let us know if you find any mistakes. Your comments are welcome in the comment box.
