- Cell Line: NCI-BL2171
- Synonyms:
- NCI-BL2171
- BL2171
- Culture Type: Cell line
- Keywords: Human Epstein-Barr virus (EBV) transformed B lymphoblast cell line
- Description: Epstein-Barr virus (EBV) transformed B lymphoblast cell line established from peripheral blood of a 50-year-old, Caucasian male donor. The donor had small cell lung cancer.
- Origin:
- Organism:
- Species: Human (Homo sapiens)
- Age: 50 years
- Ethnicity: Caucasian
- Gender: Male
- Smoking habit:-
- Tissue (origin): peripheral blood
- Disease: Normal tissue
- Year of origin:-
- Organism:
- Cell line features:
- Cell Type: B lymphoblast
- Morphology: lymphoblast-like
- Growth mode: Suspension
- Life span: Infinite (continuous culture)
- Karyotype:-
- Doubling time:
- Microsatellite instability:-
- Special features: Epstein-Barr virus (EBV) transformed
- Product:-
- Soft agar colony formation assay:-
- Tumorigenicity:-
- Gene expression:
- Sequence variations:
- For information, please visit
- https://depmap.org/portal/cell_line/ACH-002372?tab=mutations
- For information, please visit
- Cell Type: B lymphoblast
- Handling and maintenance:
- Biosafety level: BSL 2 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: RPMI-1640 Medium + 10% FBS
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO2, 95% Air
- Temperature: Humidified 37°C incubator
- Subculturing:
- Subculturing method: Dilution
- Cell dissociation solution: NA
- Harvesting time: 3 x 106 viable cells/mL
- Seeding density: 1 x 105 – 1 x 106 viable cells/mL
- Recommended split ratio:-
- Medium Renewal: Add fresh medium every 3 to 4 days
- Cryopreservation:
- Freezing medium:
- Complete growth medium + 5% DMSO
- Complete growth medium + 5% DMSO
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial: 1 ml
- Freezing medium:
- Applications:
- Cancer research
- Immunology
- Availability:
- American Type Culture Collection (ATCC): Product link
- American Type Culture Collection (ATCC): Product link
- References
- Images:
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
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