- Cell Line: SK-MEL-31
- Synonyms:
- SK-MEL-31
- SKMEL-31
- SKMEL31
- SKMel31
- SK-Mel31
- SK Mel 31
- Sk-Mel-31
- Culture Type: Cell line
- Keywords: Human Melanoma Cell Line
- Description: Established from skin tissue derived from a 33-year-old, Caucasian female patient with malignant melanoma
- Origin:
- Cell line features:
- Cell Type: Epithelial
- Morphology: Epithelial-like
- Growth mode: Adherent
- Life span: Infinite (continuous culture)
- Doubling time:-
- Karyotype: hypotetraploid or hypertriploid, Modal chromosome number : 82
- Microsatellite instability: Stable
- Special features: Non-pigmented (Cellosaurus)
- Product:-
- Soft agar colony formation assay:-
- Tumorigenicity: Yes, in nude mice, forms malignant melanoma (ATCC)
- Gene expression:-
- Sequence variations:
- BRAF (V600E mutation, Oncogenic, Gain-of-function, Heterozygous)
- For more information, please visit:
- https://depmap.org/portal/cell_line/ACH-000640?tab=mutations
- https://depmap.org/portal/cell_line/ACH-000640?tab=fusions
- Cell Type: Epithelial
- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: Minimum essential medium (Eagle, 2 mM L-glutamine) + Earle’s BSS (1.5 g/L sodium bicarbonate + 0.1 mM non-essential amino acids + 1.0 mM sodium pyruvate) + 15% FBS
- Culture conditions:
- Temperature: 37°C incubator
- Atmosphere: 95% Air, 5% CO2
- Temperature: 37°C incubator
- Subculturing:
- Subculturing method: Enzymatic dissociation
- Cell dissociation solution: 0.25% (w/v) Trypsin- 0.53 mM EDTA solution
- Harvesting time:
- Seeding density:-
- Split ratio: 1:4 to 1:6
- Split frequency:-
- Medium Renewal: 2 to 3 times per week
- Subculturing method: Enzymatic dissociation
- Cryopreservation:
- Freezing medium: Complete growth medium + 5% (v/v) DMSO
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial: 1 ml
- Applications:
- 3D cell culture
- Cancer research
- Cell Line Availability: The cell line can be obtained from the following sources.
- American Type Culture Collection (ATCC): Product link
- American Type Culture Collection (ATCC): Product link
- References
- Images:
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
- Article Authenticity Index *
- Please let us know if you find any mistakes. Your comments are welcome in the comment box.
