| CELL LINE ORIGIN | |
| Organism | Rat (Rattus norvegicus), 16 months old SD female |
| Source/Tissue | Liver |
| Disease | Hepatoma |
| Year of origin | 1988 |
| CELL LINE FEATURES | |
| Morphology | Rounded |
| Cell type | |
| Growth mode | Suspension, Grown as single cells |
| Doubling time | 29 h |
| Life span | Infinite (continuous culture) |
| Transformed | ? |
| Tumorigenic | ? |
| Drug resistance | ? |
| Products | ? |
| Karyotype | ? |
| GENE EXPRESSION | |
| APPLICATIONS | |
| CULTURE MAINTENANCE AND STORAGE | |
| Culture conditions and handling | |
| Biosafety Level | 1 REF (It may differ in your country, follow the guidelines of your institute/country) |
| Plating efficiency | ? |
| Temperature | 37°C |
| Atmosphere | Humidified incubator, 95% air + 5% CO2 |
| Base medium | DMEM |
| Complete culture medium | DMEM + 10% heat-inactivated FBS |
| Medium Renewal | |
| Subculturing procedure | |
| Harvesting time | |
| Split method | Dilution |
| Split ratio | 1:2 to 1:3 |
| Seeding density | |
| Split frequency | twice per week |
| Cryopreservation | |
| Cryomedium | Culture medium + 20% heat-inactivated FBS + 10% DMSO |
| Storage conditions | Liquid nitrogen vapor phase |
| AVAILABILITY / SOURCE | |
American Type Culture Collection (ATCC) | |
Public Health England Culture Collections | |
Sigma-Aldrich | |
Japanese Collection of Research Bioresources (JCRB) | |
DSMZ | Product page link |
Cell Bank Australia | |
ACCEGEN BIOTECHNOLOGY | Product page link |
Abcam | |
ThermoFisher Scientific | |
| CELL LINE IDENTIFICATIONS | |
| Synonyms | |
| Remarks | |
| Related cell lines | |
References
- https://www.dsmz.de/collection/catalogue/details/culture/ACC-208
