| CELL LINE ORIGIN | |
| Organism | Mouse (Mus musculus) |
| Strain | 129/Sv, BDF1,C57BL/6J |
| Age | Newborn |
| Source/Tissue | Brain, Neural |
| Disease | ? |
| Year of origin | 1999 |
| CELL LINE FEATURES | |
| Morphology | Fibroblast-like |
| Cell type | ? |
| Growth mode | Adherent, Monolayer |
| Doubling time | ? |
| Life span | Infinite (continuous culture) |
| Transformed | ? |
| Tumorigenic | ? |
| Drug resistance | ? |
| Products | ? |
| Karyotype | ? |
| GENE EXPRESSION | |
| p53-deficient cell line | |
| APPLICATIONS | |
| Used for preparation of conditioned medium for MG5 cells | |
| CULTURE MAINTENANCE AND STORAGE | |
| Culture conditions and handling | |
| Biosafety Level | 1 (It may differ in your country, follow the guidelines of your institute/country) |
| Plating efficiency | ? |
| Temperature | 37°C |
| Atmosphere | Humidified incubator, 95% air + 5% CO2 |
| Base medium | DMEM (high glucose) |
| Complete culture medium | DMEM + 10% FBS |
| Medium Renewal | ? |
| Subculturing procedure | |
| Harvesting time | Subconfluent culture |
| Split method | Trypsin/EDTA (0.25% trypsin and 0.02% EDTA in Ca, Mg-free PBS) |
| Split ratio | |
| Seeding density | |
| Split frequency | |
| Cryopreservation | |
| Cryomedium | Culture medium + 20% FBS + 10% DMSO |
| Storage conditions | Liquid nitrogen vapor phase |
| AVAILABILITY / SOURCE | |
American Type Culture Collection (ATCC) | |
Public Health England Culture Collections | |
Sigma-Aldrich | |
Japanese Collection of Research Bioresources (JCRB) | |
Cell Bank Australia | |
Nibiohn | Product page link |
ACCEGEN BIOTECHNOLOGY | |
Abcam | |
ThermoFisher Scientific | |
| CELL LINE IDENTIFICATIONS | |
| Synonyms | |
| Remarks | |
| Related cell lines | |
References
- https://cellbank.nibiohn.go.jp/~cellbank/en/search_res_det.cgi?RNO=IFO50519
