- Synonyms:
- HCC1569
- HCC-1569
- Hamon Cancer Center 1569
- Culture Type: Cell line
- Keywords: Human mammary gland epithelial-like ER negative HER2 positive invasive ductal carcinoma cell line
- Description: established from a 70-year-old, Black, female patient with metaplastic Carcinoma; TNM Stage IV, grade 3
- Origin:
- Cell line features:
- Morphology: Epithelial-like
- Cell Type: Epithelial cell
- Growth mode: adherent and suspension (mixed)
- Life span: Infinite (continuous culture)
- Karyotype:-
- Doubling time:-
- Clinically relevant gene expression:
- her2/neu positive (oncogene)
- p53 negative (oncogene)
- Epithelial glycoprotein 2 (EGP2) positive (epithelial cell-specific marker)
- Cytokeratin 19 positive (epithelial cell-specific marker)
- Estrogen receptor (ER) negative
- Progesterone receptor (PR) negative
- Sequence variations (gene mutations/deletions/fusions/translocations):
- Morphology: Epithelial-like
- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: RPMI-1640 + 10% FBS
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO2, 95% Air
- Temperature: Humidified 37°C incubator
- Subculturing:
- Subculturing method: Enzymatic dissociation
- Cell dissociation solution: 0.25% (w/v) Trypsin- 0.53 mM EDTA solution
- Harvesting time: Sub-confluent cultures
- Split ratio: 1:4 to 1:6
- Seeding densities:-
- Medium Renewal: Every 2 to 3 days
- Cryopreservation:
- Freezing medium:
- Complete growth medium + 5% DMSO (ATCC)
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density): 2 – 4 x 106 viable cells/ml
- Volume per vial: 1 ml
- Freezing medium:
- Applications:
- Cancer research
- 3D cell culture
- Availability
- American Type Culture Collection (ATCC): Product link
- References
- https://www.atcc.org/products/crl-2330
- https://www.cellosaurus.org/CVCL_1255
- https://depmap.org/portal/cell_line/ACH-000930?tab=overview
- Gazdar et al., 1998. Characterization of paired tumor and non-tumor cell lines established from patients with breast cancer. Int. J. Cancer 78: 766-774, 1998. PMID-9833771; Full Text: Wiley (Download PDF)
- Ahmadian et al., 1997. Analysis of the FHIT gene and FRA3B region in sporadic breast cancer, preneoplastic lesions, and familial breast cancer probands. Cancer Res. 57: 3664-3668. PMID-9288768; Full Text: Silverchair, Researchgate (Download PDF)
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
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