- Synonyms:
- HCC-1143
- Hamon Cancer Center 1144
- Culture Type: Cell line
- Keywords: Human mammary gland epithelial-like invasive ductal carcinoma cell line
- Description: established from a 52-year-old, white female patient with ductal carcinoma TMN Stage IIA, grade 3
- Origin:
- Cell line features:
- Morphology: Epithelial-like
- Cell Type: Epithelial cell
- Growth mode: Adherent, monolayer
- Life span: Infinite (continuous culture)
- Karyotype: hypo-tetraploid or hyper-triploid karyotype
- Doubling time: 40 – 60 h
- Clinically relevant gene expression
- her2/neu negative (oncogene)
- p53 positive (oncogene)
- Epithelial glycoprotein 2 (EGP2) positive (epithelial cell-specific marker)
- Cytokeratin 19 positive (epithelial cell-specific marker)
- Estrogen receptor (ER) negative
- Progesterone receptor (PR) negative
- Sequence variations (gene mutations/deletions/fusions/translocations):
- FGFR2 (R203C mutation)
- TP53 (R248Q mutation) (cellosaurus)
- For more information, please visit
- Morphology: Epithelial-like
- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: RPMI-1640 + 10% FBS
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO2, 95% Air
- Temperature: Humidified 37°C incubator
- Subculturing:
- Subculturing method: Enzymatic dissociation
- Cell dissociation solution: 0.25% (w/v) Trypsin- 0.53 mM EDTA solution
- Harvesting time: Near-confluent culture
- Split ratio: 1:2 to 1:4
- Seeding densities: 1-2 x 106 cells/75 cm2 flask
- Medium Renewal: Every 2 to 3 days
- Cryopreservation:
- Applications:
- Cancer research
- 3D cell culture
- Availability
- American Type Culture Collection (ATCC): Product link
- DSMZ-German Collection of Microorganisms and Cell Cultures GmbH: Product link
- References
- https://www.atcc.org/products/crl-2321
- https://www.dsmz.de/collection/catalogue/details/culture/ACC-517
- https://www.cellosaurus.org/CVCL_1245
- https://depmap.org/portal/cell_line/ACH-000374?tab=overview
- Gazdar et al., 1998. Characterization of paired tumor and non-tumor cell lines established from patients with breast cancer. Int. J. Cancer 78: 766-774, 1998. PMID-9833771; Full Text: Wiley (Download PDF)
- Chavez et al., 2010. Triple negative breast cancer cell lines: one tool in the search for better treatment of triple negative breast cancer. Breast Dis. 32(1-2), 35-48. PMID-21778573; Full Text: iospress, PMC
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
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- Please let us know if you find any mistakes. Your comments are welcome in the comment box.
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