Posted in Database: Cell Line

Cell Line: PANC-1 Ecadherin EmGFP

   January 28, 2024  Leave a Comment on Cell Line: PANC-1 Ecadherin EmGFP

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  • Cell Line: PANC-1 Ecadherin EmGFP
  • Synonyms:
    • Panc1-Ecadherin EmGFP
    • PANC1-PANC-1 Ecadherin EmGFP
    • PANC-1 ECAD-EmGFP
  • Culture Type: Cell line
  • Keywords: ECAD-EmGFP reporter cell line
  • Description: 
    • Parent line: PANC-1
    • Created by tagging green fluorescent protein (EmGFP) to the C-terminal of the E-cadherin gene using CRISPR/Cas9 gene editing system
  • Origin:
    • Organism: 
      • Species: Human (Homo sapiens)
      • Age: 56 years
      • Ethnicity: White
      • Gender: Male
      • Tissue: Pancreas
      • Disease: Pancreatic adenocarcinoma
        • Molecular subtype: Exocrine Adenocarcinoma (depmap)
        • Primary/Metastasis: Primary (depmap)
    • Year of origin:-
  • Cell line features:
    • Cell Type:-
    • Morphology: Fibroblast-like
    • Growth mode: Adherent
    • Life span: Infinite (continuous culture)
    • Karyotype:-
    • Doubling time:-
    • Tumorigenicity:-
  • Handling and maintenance:
    • Biosafety level: BSL 2 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
    • Complete culture medium: DMEM + 10% FBS
    • Culture conditions: 
      • Temperature: Humidified 37°C incubator
      • Atmosphere: 5% CO2, 95% Air
    • Subculturing:
      • Subculturing method: Enzymatic dissociation
      • Cell dissociation solution: 0.25% (w/v) Trypsin-0.53 mM EDTA solution
      • Harvesting time: Sub-confluent culture
      • Split ratio: 1:3 to 1:8
      • Medium Renewal: Every 2 to 3 days.
    • Cryopreservation:
      • Freezing medium: Complete growth medium + 5% DMSO
      • Storage conditions: Vapor phase of liquid nitrogen
      • Cell suspension (cell density): 2 – 5 x 106 viable cells/mL
      • Volume per vial: 1 mL
  • Applications: 
    • Cancer research
    • Mesenchymal to epithelial transition studies
    • Anti-EMT/MET drug screening
    • Pancreatic cancer drug screening
  • Availability:
  • References:
    • ATCC
    • Cellosaurus
    • Lieber et al., 1975. Establishment of a continuous tumor-cell line (panc-1) from a human carcinoma of the exocrine pancreas. Int. J. Cancer 15: 741-747. PMID-1140870
  • Images:-
  • Abbreviations
    • BSL (Biosafety level)
    • FBS (Fetal Bovine Serum)
    • DMSO (Dimethyl sulfoxide)
    • DMEM (Dulbecco’s Modified Eagle’s Medium)
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