- Cell Line: PANC-1 Ecadherin EmGFP
- Synonyms:
- Panc1-Ecadherin EmGFP
- PANC1-PANC-1 Ecadherin EmGFP
- PANC-1 ECAD-EmGFP
- Culture Type: Cell line
- Keywords: ECAD-EmGFP reporter cell line
- Description:
- Parent line: PANC-1
- Created by tagging green fluorescent protein (EmGFP) to the C-terminal of the E-cadherin gene using CRISPR/Cas9 gene editing system
- Origin:
- Cell line features:
- Cell Type:-
- Morphology: Fibroblast-like
- Growth mode: Adherent
- Life span: Infinite (continuous culture)
- Karyotype:-
- Doubling time:-
- Tumorigenicity:-
- Handling and maintenance:
- Biosafety level: BSL 2 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: DMEM + 10% FBS
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO2, 95% Air
- Temperature: Humidified 37°C incubator
- Subculturing:
- Subculturing method: Enzymatic dissociation
- Cell dissociation solution: 0.25% (w/v) Trypsin-0.53 mM EDTA solution
- Harvesting time: Sub-confluent culture
- Split ratio: 1:3 to 1:8
- Medium Renewal: Every 2 to 3 days.
- Cryopreservation:
- Freezing medium: Complete growth medium + 5% DMSO
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density): 2 – 5 x 106 viable cells/mL
- Volume per vial: 1 mL
- Freezing medium: Complete growth medium + 5% DMSO
- Applications:
- Cancer research
- Mesenchymal to epithelial transition studies
- Anti-EMT/MET drug screening
- Pancreatic cancer drug screening
- Availability:
- American Type Culture Collection (ATCC): Product link
- References:
- ATCC
- Cellosaurus
- Lieber et al., 1975. Establishment of a continuous tumor-cell line (panc-1) from a human carcinoma of the exocrine pancreas. Int. J. Cancer 15: 741-747. PMID-1140870
- Images:-
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
- DMEM (Dulbecco’s Modified Eagle’s Medium)
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