- Cell Line: Panc 04.03
- Synonyms:
- PANC0403
- Panc0403
- Culture Type: Cell line
- Keywords: Human Pancreatic Adenocarcinoma Epithelial Cell Line
- Description: Established from a primary tumor taken from the pancreas of a 70-year-old Caucasian male patient with pancreatic adenocarcinoma
- Origin:
- Organism:
- Species: Human (Homo sapiens)
- Age: 70 years
- Ethnicity: Caucasian
- Gender: Female
- Tissue: Pancreas
- Disease: Pancreatic adenocarcinoma
- Molecular subtype: Exocrine Adenocarcinoma
- Primary/Metastasis: Primary
- Molecular subtype: Exocrine Adenocarcinoma
- Year of origin: 1995
- Organism:
- Cell line features:
- Cell Type: Epithelial cell
- Morphology: Epithelial-like
- Growth mode: Adherent
- Life span: Infinite (continuous culture)
- Karyotype:-
- Doubling time: ≈ 50.5 h
- Tumorigenicity: forms tumors in nude or SCID mice
- Gene expression:
- Positive for K-ras
- Positive for MHC class I
- Negative for MHC class II
- Positive for Cytokeratin 7
- Positive for Cytokeratin 18
- Sequence variations:
- KRAS (G12D mutation, Oncogenic, Gain-of-function)
- TP53 (G245S mutation, Oncogenic, Loss-of-function)
- For more information, please visit
- https://depmap.org/portal/cell_line/ACH-000235?tab=mutations
- https://depmap.org/portal/cell_line/ACH-000235?tab=fusions
- https://depmap.org/portal/cell_line/ACH-000235?tab=translocations
- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: RPMI-1640 Medium + 20 Units/ml human recombinant insulin + 15% FBS
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO2, 95% Air
- Temperature: Humidified 37°C incubator
- Subculturing:
- Subculturing method: Enzymatic dissociation
- Cell dissociation solution: 0.25% (w/v) Trypsin-0.53 mM EDTA solution
- Harvesting time:-
- Split ratio: 1:2 to 1:3
- Medium Renewal: Every 2 to 3 days.
- Cryopreservation:
- Freezing medium: Complete growth medium + 5% DMSO
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial:-
- Freezing medium: Complete growth medium + 5% DMSO
- Applications:
- Cancer research
- 3D cell culture
- Cancer research
- Availability:
- American Type Culture Collection (ATCC): Product link
- References:
- ATCC
- Cellosaurus
- DepMap
- Jaffee et al., 1998. Development and characterization of a cytokine-secreting pancreatic adenocarcinoma vaccine from primary tumors for use in clinical trials. Cancer J. Sci. Am. 4: 194-203. PMID-9612602
- Images:-
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
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