- Cell Line: Panc 04.03
- Synonyms:
- PANC0403
- Panc0403
- Culture Type: Cell line
- Keywords: Human Pancreatic Adenocarcinoma Epithelial Cell Line
- Description: Established from a primary tumor taken from the pancreas of a 70-year-old Caucasian male patient with pancreatic adenocarcinoma
- Origin:
- Organism:
- Species: Human (Homo sapiens)
- Age: 70 years
- Ethnicity: Caucasian
- Gender: Female
- Tissue: Pancreas
- Disease: Pancreatic adenocarcinoma
- Molecular subtype: Exocrine Adenocarcinoma
- Primary/Metastasis: Primary
- Molecular subtype: Exocrine Adenocarcinoma
- Year of origin: 1995
- Organism:
- Cell line features:
- Cell Type: Epithelial cell
- Morphology: Epithelial-like
- Growth mode: Adherent
- Life span: Infinite (continuous culture)
- Karyotype:-
- Doubling time: ≈ 50.5 h
- Tumorigenicity: forms tumors in nude or SCID mice
- Gene expression:
- Positive for K-ras
- Positive for MHC class I
- Negative for MHC class II
- Positive for Cytokeratin 7
- Positive for Cytokeratin 18
- Sequence variations:
- KRAS (G12D mutation, Oncogenic, Gain-of-function)
- TP53 (G245S mutation, Oncogenic, Loss-of-function)
- For more information, please visit
- https://depmap.org/portal/cell_line/ACH-000235?tab=mutations
- https://depmap.org/portal/cell_line/ACH-000235?tab=fusions
- https://depmap.org/portal/cell_line/ACH-000235?tab=translocations
- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: RPMI-1640 Medium + 20 Units/ml human recombinant insulin + 15% FBS
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO2, 95% Air
- Temperature: Humidified 37°C incubator
- Subculturing:
- Subculturing method: Enzymatic dissociation
- Cell dissociation solution: 0.25% (w/v) Trypsin-0.53 mM EDTA solution
- Harvesting time:-
- Split ratio: 1:2 to 1:3
- Medium Renewal: Every 2 to 3 days.
- Cryopreservation:
- Freezing medium: Complete growth medium + 5% DMSO
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial:-
- Freezing medium: Complete growth medium + 5% DMSO
- Applications:
- Cancer research
- 3D cell culture
- Cancer research
- Availability:
- American Type Culture Collection (ATCC): Product link
- References:
- ATCC
- Cellosaurus
- DepMap
- Jaffee et al., 1998. Development and characterization of a cytokine-secreting pancreatic adenocarcinoma vaccine from primary tumors for use in clinical trials. Cancer J. Sci. Am. 4: 194-203. PMID-9612602
- Images:-
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
- Article Authenticity Index *
- Please let us know if you find any mistakes. Your comments are welcome in the comment box.
Was this post helpful?
Let us know if you liked the post. That’s the only way we can improve.
