- Cell Line: NCI-H1385
- Synonyms:
- NCIH1385
- NCI-H1385
- H1385
- H-1385
- Culture Type: Cell line
- Keywords: Human Non-Small Cell Lung Carcinoma Epithelial Cell Line
- Description: Established from the metastatic tumor (lymph node) of a 49-year-old, Black female patient with Non-small cell lung squamous cell carcinoma, stage 3A
- Origin:
- Organism:
- Species: Human (Homo sapiens)
- Age: 49 years
- Ethnicity: Black (African American)
- Gender: Female
- Tissue (origin): Lung
- Tissue (isolation): Lymph node (metastatic site)
- Disease:
- Lung Squamous Cell Carcinoma
- Molecular subtype: Non-small cell lung squamous cell carcinoma, stage 3A
- Molecular subtype: Non-small cell lung squamous cell carcinoma, stage 3A
- Primary/Metastasis: Metastasis
- Lung Squamous Cell Carcinoma
- Year of origin: 1986
- Organism:
- Cell line features:
- Cell Type:-
- Morphology:-
- Growth mode: Suspension
- Life span: Infinite (continuous culture)
- Karyotype:-
- Doubling time:-
- Microsatellite instability:-
- Special features:-
- Product:-
- Tumorigenicity:-
- Gene expression:-
- Sequence variations:
- Cell Type:-
- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: DMEM:FI2 + 1X ACL4 Cocktail (0.02 mg/ml Human lnsulin + 25 nM Sodium Selenite + 10 uM Ethanolamine + 10 uM Phosphorylethanolamine + 100 pM Triiodo-L-thyronine + 5 g/mL Bovine Serum Albumin + 0.5 mM Sodium Pyruvate + 10 mM HEPES + 2 mM L-glutamine + 50 nM Hydrocortisone) + 5 g/ml Bovine Serum Albumin + 0.01 mg/ml Transferrin + l ng/mL EGF (ATCC)
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO2, 95% Air
- Temperature: Humidified 37°C incubator
- Subculturing:
- Subculturing method: Dilution
- Harvesting time:-
- Split ratio:-
- Medium Renewal: 2 times per week
- Cryopreservation:
- Freezing medium: Complete growth medium + 5% DMSO
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial:-
- Applications:
- Cancer research
- 3D cell culture
- Availability
- American Type Culture Collection (ATCC): Product link
- Cyton: Product number: Product link
- References
- Images:-
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
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