- Cell Line: NCI-H226
- Synonyms:
- NCIH226
- NCI-H226
- H226
- H-226
- Culture Type: Cell line
- Keywords: Human Lung Adenocarcinoma Epithelial Cell Line
- Description: Established from the metastatic tumor (Pleural effusion) of a caucasian, male patient with pleural epithelioid mesothelioma (Squamous Cell Carcinoma)
- Origin:
- Organism:
- Species: Human (Homo sapiens)
- Age:-
- Ethnicity: Caucasian
- Gender: Male
- Tissue (origin): Lung
- Tissue (isolation): Pleural effusion (metastatic site)
- Disease:
- Squamous Cell Carcinoma
- Pleural epithelioid mesothelioma
- Primary/Metastasis: Metastasis
- Squamous Cell Carcinoma
- Year of origin: 1980
- Organism:
- Cell line features:
- Cell Type:-
- Morphology: Epithelial-like
- Growth mode: Adherent, monolayer
- Life span: Infinite (continuous culture)
- Karyotype:-
- Doubling time:
- 43 hours (Cellosauruss)
- 52 hours (Cellosaurus)
- Microsatellite instability: Stable
- Special features:-
- Product:-
- Tumorigenicity:-
- Gene expression:-
- Sequence variations:
- Cell Type:-
- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: RPMI-1640 Medium + 10% FBS
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO2, 95% Air
- Temperature: Humidified 37°C incubator
- Subculturing:
- Subculturing method: Enzymatic dissociation
- Cell dissociation solution: 0.25% (w/v) Trypsin-0.53 mM EDTA solution
- Harvesting time:-
- Split ratio: 1:4 to 1:8
- Medium Renewal: Every 2 to3 days
- Cryopreservation:
- Freezing medium: Complete growth medium + 5% DMSO
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial:-
- Applications:
- Cancer research
- 3D cell culture
- Availability
- American Type Culture Collection (ATCC): Product link
- Cyton: Product number: Product link
- References
- Images:-
- NCI-H226 cell image link (Source: Cytion)
- NCI-H226 cell image link (Source: Cytion)
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
- Article Authenticity Index *
- Please let us know if you find any mistakes. Your comments are welcome in the comment box.
Was this post helpful?
Let us know if you liked the post. That’s the only way we can improve.
