- Cell Line: NCI-H226
- Synonyms:
- NCIH226
- NCI-H226
- H226
- H-226
- Culture Type: Cell line
- Keywords: Human Lung Adenocarcinoma Epithelial Cell Line
- Description: Established from the metastatic tumor (Pleural effusion) of a caucasian, male patient with pleural epithelioid mesothelioma (Squamous Cell Carcinoma)
- Origin:
- Organism:
- Species: Human (Homo sapiens)
- Age:-
- Ethnicity: Caucasian
- Gender: Male
- Tissue (origin): Lung
- Tissue (isolation): Pleural effusion (metastatic site)
- Disease:
- Squamous Cell Carcinoma
- Pleural epithelioid mesothelioma
- Primary/Metastasis: Metastasis
- Squamous Cell Carcinoma
- Year of origin: 1980
- Organism:
- Cell line features:
- Cell Type:-
- Morphology: Epithelial-like
- Growth mode: Adherent, monolayer
- Life span: Infinite (continuous culture)
- Karyotype:-
- Doubling time:
- 43 hours (Cellosauruss)
- 52 hours (Cellosaurus)
- Microsatellite instability: Stable
- Special features:-
- Product:-
- Tumorigenicity:-
- Gene expression:-
- Sequence variations:
- Cell Type:-
- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: RPMI-1640 Medium + 10% FBS
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO2, 95% Air
- Temperature: Humidified 37°C incubator
- Subculturing:
- Subculturing method: Enzymatic dissociation
- Cell dissociation solution: 0.25% (w/v) Trypsin-0.53 mM EDTA solution
- Harvesting time:-
- Split ratio: 1:4 to 1:8
- Medium Renewal: Every 2 to3 days
- Cryopreservation:
- Freezing medium: Complete growth medium + 5% DMSO
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial:-
- Applications:
- Cancer research
- 3D cell culture
- Availability
- American Type Culture Collection (ATCC): Product link
- Cyton: Product number: Product link
- References
- Images:-
- NCI-H226 cell image link (Source: Cytion)
- NCI-H226 cell image link (Source: Cytion)
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
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