- Cell Line: NCI-H69
- Synonyms:
- NCIH69
- NCI-H69
- H60
- H-69
- Culture Type: Cell line
- Keywords: Human Lung Adenocarcinoma Epithelial Cell Line
- Description: Established from the pleural fluid of a 55-year-old Caucasian male patient with Small Cell Lung Carcinoma
- Origin:
- Organism:
- Species: Human (Homo sapiens)
- Age: 55 years
- Ethnicity: Caucasian
- Gender: Male
- Tissue (origin): Lung
- Tissue (isolation): Pleural fluid (metastatic site)
- Disease:
- Small Cell Lung Carcinoma
- Primary/Metastasis: Metastasis
- Year of origin:-
- Organism:
- Cell line features:
- Cell Type:-
- Morphology:
- Growth mode: Suspension, multicellular aggregates
- Life span: Infinite (continuous culture)
- Karyotype: Modal number = 76 to 78, range = 40 to 87
- Doubling time: 56.0 hours
- Microsatellite instability: Stable
- Special features:-
- Product: Insulin-like growth factor II (IGF II)
- Tumorigenicity: Yes, in nude mice
- Note: Form colonies in soft agar
- Gene expression:
- Positive for myc (oncogene)
- Positive for myb (oncogene)
- Positive for fes (oncogene)
- Positive for fms (oncogene)
- Positive for raf (oncogene)
- Positive for ras (oncogene)
- Positive for cytokeratins
- Positive for myc (oncogene)
- Sequence variations:
- N-myc gene amplification
- For more information, please visit
- N-myc gene amplification
- Cell Type:-
- Handling and maintenance:
- Biosafety level:
- BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- BSL 2 (Culturecollections)
- Complete culture medium:
- RPMI-1640 Medium + 10% FBS (ATCC)
- RPMI 1640 + 2mM Glutamine + 10% FBS (Culturecollections)
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO2, 95% Air
- Temperature: Humidified 37°C incubator
- Subculturing:
- Subculturing method: shaking and dilution
- Maintain cultures between 6 x 104 to 5 x 105 cells/ml
- Harvesting time:-
- Split ratio: 1:2 to 1:4
- Subculturing method: shaking and dilution
- Cryopreservation:
- Freezing medium: Complete growth medium + 5% DMSO
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial:-
- Biosafety level:
- Applications:
- Cancer research
- 3D cell culture
- Availability
- American Type Culture Collection (ATCC): Product link
- UK Health Security Agency Culture Collections: Product link
- Cyton: Product number: Product link
- References
- Images:-
- NCI-H69 cell image link (Source: ATCC)
- NCI-H69 cell image link (Source: Culture Collections)
- NCI-H69 cell image link (Source: Cytion)
- NCI-H69 cell image link (Source: Cytion)
- NCI-H69 cell image link (Source: Cytion)
- NCI-H69 cell image link (Source: Cytion)
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
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