- Cell Line: NCI-H526
- Synonyms:
- NCI-H526
- NCIH526
- H526
- H-526
- Culture Type: Cell line
- Keywords: Human Small Cell Lung Carcinoma Epithelial Cell Line
- Description: Established from the Bone marrow (metastatic site) of a 55-year-old, Caucasian male patient with Small Cell Lung Carcinoma.
- Origin:
- Organism:
- Species: Human (Homo sapiens)
- Age: 55 years
- Ethnicity: Caucasian
- Gender: Male
- Tissue (origin): Lung
- Tissue (isolation): Bone marrow (metastatic site)
- Disease:
- Small Cell Lung Carcinoma
- Primary/Metastasis: Metastatic
- Small Cell Lung Carcinoma
- Year of origin:-
- Organism:
- Cell line features:
- Cell Type: Epithelial cell
- Morphology: Round clusters
- Growth mode: Suspension
- Life span: Infinite (continuous culture)
- Karyotype:-
- Doubling time:-
- Microsatellite instability: Instable
- Special features:-
- Product:-
- Tumorigenicity:- Yes, cells produce tumors in athymic nude mice
- Gene expression:
- Expression of the oncogenes myc, myb, fes, fms, raf, ras has been reported.
- Expression of Insulin-like growth factor II (IGF II) and bombesin has been reported.
- Elevated levels of neuron-specific enolase and the brain isoenzyme of creatine kinase have been reported.
- No expression of L-dopa carboxylase has been reported.
- Expression of c-kit gene as well as the N-myc gene, but not c-myc or L-myc has been reported
- Expression of proto-oncogenes N-ras, Ki-ras, Ha-ras, and c-raf1 has been reported
- Sequence variations:
- Amplification of N-myc has been reported
- For information, please visit
- Cell Type: Epithelial cell
- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: RPMI-1640 Medium + 10% FBS
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO2, 95% Air
- Temperature: Humidified 37°C incubator
- Subculturing:
- Subculturing method: Dilution (medium replacement or addition of fresh medium following centrifugation)
- Harvesting time:-
- Split ratio:
- Medium Renewal: Every 2 to 3 days
- Subculturing method: Dilution (medium replacement or addition of fresh medium following centrifugation)
- Cryopreservation:
- Freezing medium: Complete growth medium + 5% DMSO
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial:-
- Applications:
- Cancer research
- 3D cell culture
- Availability
- American Type Culture Collection (ATCC): Product link
- American Type Culture Collection (ATCC): Product link
- References
- Images:
- NCI-H526 cell image link (Source: ATCC)
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
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