- Cell Line: NCI-H1048
- Synonyms:
- NCI-H1048
- NCIH1048
- H1048
- H-1048
- Culture Type: Cell line
- Keywords: Human Small Cell Lung Carcinoma Epithelial Cell Line
- Description: Established from the pleural effusion (metastatic site) of a 53-year-old, African American female patient with Small Cell Lung Carcinoma (Neuroendocrine Tumor)
- Origin:
- Organism:
- Species: Human (Homo sapiens)
- Age: 53 years
- Ethnicity: African American
- Gender: Female
- Tissue (origin): Lung
- Tissue (isolation): Pleural effusion (metastatic site)
- Disease:
- Small Cell Lung Carcinoma/Lung Neuroendocrine Tumor
- Primary/Metastasis: Metastatic
- Small Cell Lung Carcinoma/Lung Neuroendocrine Tumor
- Year of origin: 1985
- Organism:
- Cell line features:
- Cell Type:-
- Morphology: Epithelial-like
- Growth mode: Adherent
- Life span: Infinite (continuous culture)
- Karyotype:-
- Doubling time:-
- Microsatellite instability: Instable
- Special features:-
- Product:-
- Tumorigenicity:-
- Gene expression:-
- Sequence variations:
- TP53 (R273C mutation, Likely Oncogenic, Likely Loss-of-function, Heterozygous)
- PIK3CA (H1047R mutation, Oncogenic Gain-of-function, Heterozygous)
- PIK3CA (K111R mutation, Likely Oncogenic, Likely Gain-of-function)
- RRAS2 (G23S mutation, Likely Oncogenic, Likely Gain-of-function)
- For information, please visit
- Cell Type:-
- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: DMEM:F12 Medium (Catalog No.30-2006) + 0.005 mg/ml Insulin + 0.01 mg/ml Transferrin + 30nM Sodium selenite (final conc.) + 10 nM Hydrocortisone (final conc.) + 10 nM beta-estradiol (final conc.) + extra 2mM L-glutamine (for final conc. of 4.5 mM) + 5% fetal bovine serum (final conc.)
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO2, 95% Air
- Temperature: Humidified 37°C incubator
- Subculturing:
- Subculturing method: Enzymatic dissociation
- Cell dissociation solution: 0.25% (w/v) Trypsin-0.53 mM EDTA solution
- Harvesting time:-
- Split ratio: 1:3 to 1:8
- Medium Renewal:-
- Cryopreservation:
- Freezing medium: Complete growth medium + 5% DMSO
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial:-
- Applications:
- Cancer research
- 3D cell culture
- Availability
- American Type Culture Collection (ATCC): Product link
- AddexBio Technologies: Product link
- References
- Images:
- NCI-H1048 cell image link (Source: ATCC)
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
- Article Authenticity Index *
- Please let us know if you find any mistakes. Your comments are welcome in the comment box.
Was this post helpful?
Let us know if you liked the post. That’s the only way we can improve.
