- Cell Line: NCI-H1373
- Synonyms:
- NCI-H1373
- NCIH1373
- H1373
- H-1373
- Culture Type: Cell line
- Keywords: Human Lung Adenocarcinoma Cell Line
- Description: Established from the primary lung tumor of a 56-year-old, Black male with lung adenocarcinoma
- Origin:
- Organism:
- Species: Human (Homo sapiens)
- Age: 56 years
- Ethnicity: Black
- Gender: Male
- Tissue (origin): Lung
- Disease:
- Lung adenocarcinoma
- Primary/Metastasis: Primary
- Year of origin: 1986
- Organism:
- Cell line features:
- Cell Type:-
- Morphology: Epithelial-like
- Growth mode: Adherent
- Life span: Infinite (continuous culture)
- Karyotype: Modal number = 60; range = 52-118
- Doubling time: 36.7 hours
- Microsatellite instability:-
- Special features:-
- Product:-
- Tumorigenicity:-
- Gene expression:
- Sequence variations:
- KRAS (G12C (c.34G>T) mutation, Oncogenic, Gain-of-function, Homozygous)
- For information, please visit
- Cell Type:-
- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: DMEM:F-12 + 10% FBS
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO2, 95% Air
- Temperature: Humidified 37°C incubator
- Subculturing:
- Subculturing method: Enzymatic dissociation
- Cell dissociation solution: 0.25% (w/v) Trypsin-0.53 mM EDTA solution
- Harvesting time:-
- Split ratio: 1:3 to 1:4
- Medium Renewal: 2 to 3 times per week
- Cryopreservation:
- Freezing medium: Complete growth medium + 5% DMSO
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial:
- Applications:
- Cancer research
- 3D cell culture
- Availability:
- American Type Culture Collection (ATCC): Product link
- References
- Images:
- NCI-H1373 cell image link (Source: DepMap)
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
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