- Cell Line: NCI-H1355
- Synonyms:
- NCI-H1355
- NCIH1355
- H1355
- H-1355
- Culture Type: Cell line
- Keywords: Human Lung Adenocarcinoma Cell Line
- Description: Established from pleural effusion metastasis of a 53-year-old, Caucasian male with stage 4 lung adenocarcinoma.
- Origin:
- Organism:
- Species: Human (Homo sapiens)
- Age: 53 years
- Ethnicity: Caucasian
- Gender: Male
- Tissue (origin): Lung
- Tissue (isolation): Pleural effusion (metastatic site)
- Disease:
- Non-Small Cell Lung Cancer
- Lung Adenocarcinoma
- Lung Adenocarcinoma
- Primary/Metastasis: Metastatic
- Non-Small Cell Lung Cancer
- Year of origin: 1986
- Organism:
- Cell line features:
- Cell Type:-
- Morphology:
- Growth mode: Suspension
- Life span: Infinite (continuous culture)
- Karyotype: Modal number = 54, range = 43-66
- Doubling time: 48.8 hours (Cellosaurus)
- Microsatellite instability: Stable
- Special features:-
- Product:-
- Tumorigenicity:-
- Gene expression:-
- Sequence variations:
- KRAS (G13C mutation, Oncogenic, Gain-of-function, Heterozygous)
- TP53 (E285K mutation, Likely Oncogenic Likely Loss-of-function, Homozygous)
- For information, please visit
- Cell Type:-
- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium:
- DMEM:F12 medium + supplements *
- Supplements: To make the complete growth medium, add the following components to 475 mL of the base medium: 25 mL ACL-4 20X Cocktail, 7 mL Bovine Serum Albumin (Sigma A7979); Transferrin, 1 mg/mL stock- use 10 µL/1 mL culture medium; EGF, 1 µg/mL stock- use 1 µL/1 mL culture medium. (ATCC)
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO2, 95% Air
- Temperature: Humidified 37°C incubator
- Subculturing:
- Subculturing method: Dilution (Addition or replacement of culture medium)
- Harvesting time:-
- Split ratio:-
- Medium Renewal: twice weekly
- Subculturing method: Dilution (Addition or replacement of culture medium)
- Cryopreservation:
- Freezing medium:
- Complete growth medium + 10% (v/v) FBS + 5% (v/v) DMSO
- Complete growth medium + 10% (v/v) FBS + 5% (v/v) DMSO
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density): ≈3 x 106 cells/ml
- Volume per vial: 1 ml
- Freezing medium:
- Applications:
- Cancer research
- 3D cell culture
- Availability
- American Type Culture Collection (ATCC): Product link
- References
- Images:
- NCI-H1355 image link (Source: ATCC)
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
- Article Authenticity Index *
- Please let us know if you find any mistakes. Your comments are welcome in the comment box.
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