- Cell Line: NCI-H2286
- Synonyms:
- NCI-H2286
- NCIH2286
- H2286
- H-2286
- Culture Type: Cell line
- Keywords: Human Small Cell Lung Adenocarcinoma Cell line
- Description: Established from the lungs of a 57-year-old, White female patient with stage 1 small cell lung adenocarcinoma
- Origin:
- Organism:
- Species: Human (Homo sapiens)
- Age: 57
- Ethnicity: White
- Gender: Female
- Smoking habit: Smoke, 60 pack years
- Tissue (origin): Lung
- Disease:
- Small cell lung adenocarcinoma, stage 1
- Primary/Metastasis: Primary
- Small cell lung adenocarcinoma, stage 1
- Year of origin: 1990
- Organism:
- Cell line features:
- Cell Type:
- Morphology: Rounded
- Growth mode: Loosely adherent
- Life span: Infinite (continuous culture)
- Karyotype:-
Doubling time:- - Microsatellite instability:-
- Special features:
- Product:-
- Soft agar colony formation assay:-
- Tumorigenicity:-
- Gene expression:
- Sequence variations:
- STK11 (D194H mutation, Likely Oncogenic Likely Loss-of-function)
- SMARCA4 (R1135Q mutation, Likely Oncogenic Unknown)
- For information, please visit
- Cell Type:
- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: RPMI-1640 Medium + 5% FBS
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO2, 95% Air
- Temperature: Humidified 37°C incubator
- Subculturing:
- Subculturing method: Enzymatic dissociation
- Cell dissociation solution: 0.25% (w/v) Trypsin-0.53 mM EDTA solution
- Harvesting time:-
- Seeding density:-
- Split ratio: 1:2 to 1:4
- Medium Renewal: Add fresh medium twice weekly
- Cryopreservation:
- Freezing medium:
- RPMI-1640 Medium + 10% FBS + 5% DMSO
- RPMI-1640 Medium + 10% FBS + 5% DMSO
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial:
- Freezing medium:
- Applications:
- Cancer research
- 3D cell culture
- Availability:
- American Type Culture Collection (ATCC): Product link
- American Type Culture Collection (ATCC): Product link
- References
- Images:
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
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