- Cell Line: NCI-H2196
- Synonyms:
- NCI-H2196
- NCIH2196
- H2196
- H-2196
- Culture Type: Cell line
- Keywords: Human Small Cell Lung Carcinoma Cell line
- Description: Established from the bone marrow (metastatic site) of a 57-year-old, Caucasian male patient with stage E small cell lung carcinoma
- Origin:
- Organism:
- Species: Human (Homo sapiens)
- Age: 57
- Ethnicity: Caucasian
- Gender: Male
- Smoking habit: Smokeer, 100 pack years
- Tissue (origin): Lung
- Disease:
- Small cell lung Carcinoma, stage E
- Primary/Metastasis: Metastatic (bone marrow)
- Small cell lung Carcinoma, stage E
- Year of origin: 1989
- Organism:
- Cell line features:
- Cell Type:
- Morphology:
- Growth mode: Adherent
- Life span: Infinite (continuous culture)
- Karyotype:-
Doubling time:- - Microsatellite instability:-
- Special features:
- Product:-
- Soft agar colony formation assay:-
- Tumorigenicity:-
- Gene expression:
- Sequence variations:
- TP53 (V157F mutation, Likely Oncogenic Likely Loss-of-function, Homozygous)
- For information, please visit
- Cell Type:
- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: DMEM:F12 Medium (ATCC, Catalog No.30-2006) + Supplements
- Supplements:
- 0.005 mg/ml Insulin
- 0.01 mg/ml Transferrin
- 30nM Sodium selenite (final conc.)
- 10 nM Hydrocortisone (final conc.)
- 10 nM beta-estradiol (final conc.)
- extra 2mM L-glutamine (for final conc. of 4.5 mM)
- 5% fetal bovine serum (final conc.)
- Supplements:
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO2, 95% Air
- Temperature: Humidified 37°C incubator
- Subculturing:
- Subculturing method: Enzymatic dissociation
- Cell dissociation solution: 0.25% (w/v) Trypsin-0.53 mM EDTA solution
- Harvesting time:-
- Seeding density:-
- Split ratio: 1:3 to 1:6
- Medium Renewal: Every 2 to 3 days
- Cryopreservation:
- Freezing medium:
- Complete growth medium + 5% FBS + 5% DMSO
- Complete growth medium + 5% FBS + 5% DMSO
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial:
- Freezing medium:
- Applications:
- Cancer research
- 3D cell culture
- Immunology
- Availability:
- American Type Culture Collection (ATCC): Product link
- American Type Culture Collection (ATCC): Product link
- References
- Images:
- NCI-H2196 cell image link (Source: DepMap)
- NCI-H2196 cell image link (Source: DepMap)
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
- Article Authenticity Index *
- Please let us know if you find any mistakes. Your comments are welcome in the comment box.
Was this post helpful?
Let us know if you liked the post. That’s the only way we can improve.
