- Cell Line: NCI-H2196
- Synonyms:
- NCI-H2196
- NCIH2196
- H2196
- H-2196
- Culture Type: Cell line
- Keywords: Human Small Cell Lung Carcinoma Cell line
- Description: Established from the bone marrow (metastatic site) of a 57-year-old, Caucasian male patient with stage E small cell lung carcinoma
- Origin:
- Organism:
- Species: Human (Homo sapiens)
- Age: 57
- Ethnicity: Caucasian
- Gender: Male
- Smoking habit: Smokeer, 100 pack years
- Tissue (origin): Lung
- Disease:
- Small cell lung Carcinoma, stage E
- Primary/Metastasis: Metastatic (bone marrow)
- Small cell lung Carcinoma, stage E
- Year of origin: 1989
- Organism:
- Cell line features:
- Cell Type:
- Morphology:
- Growth mode: Adherent
- Life span: Infinite (continuous culture)
- Karyotype:-
Doubling time:- - Microsatellite instability:-
- Special features:
- Product:-
- Soft agar colony formation assay:-
- Tumorigenicity:-
- Gene expression:
- Sequence variations:
- TP53 (V157F mutation, Likely Oncogenic Likely Loss-of-function, Homozygous)
- For information, please visit
- Cell Type:
- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: DMEM:F12 Medium (ATCC, Catalog No.30-2006) + Supplements
- Supplements:
- 0.005 mg/ml Insulin
- 0.01 mg/ml Transferrin
- 30nM Sodium selenite (final conc.)
- 10 nM Hydrocortisone (final conc.)
- 10 nM beta-estradiol (final conc.)
- extra 2mM L-glutamine (for final conc. of 4.5 mM)
- 5% fetal bovine serum (final conc.)
- Supplements:
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO2, 95% Air
- Temperature: Humidified 37°C incubator
- Subculturing:
- Subculturing method: Enzymatic dissociation
- Cell dissociation solution: 0.25% (w/v) Trypsin-0.53 mM EDTA solution
- Harvesting time:-
- Seeding density:-
- Split ratio: 1:3 to 1:6
- Medium Renewal: Every 2 to 3 days
- Cryopreservation:
- Freezing medium:
- Complete growth medium + 5% FBS + 5% DMSO
- Complete growth medium + 5% FBS + 5% DMSO
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial:
- Freezing medium:
- Applications:
- Cancer research
- 3D cell culture
- Immunology
- Availability:
- American Type Culture Collection (ATCC): Product link
- American Type Culture Collection (ATCC): Product link
- References
- Images:
- NCI-H2196 cell image link (Source: DepMap)
- NCI-H2196 cell image link (Source: DepMap)
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
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