- Cell Line: NCI-H358
- Synonyms:
- NCI-H358
- NCIH358
- H358
- H-358
- Culture Type: Cell line
- Keywords: Human bronchioalveolar carcinoma cell line
- Description: Established from primary bronchioalveolar carcinoma of the lung from a Caucasian male
- Origin:
- Organism:
- Species: Human (Homo sapiens)
- Age:-
- Ethnicity: Caucasian
- Gender: Male
- Smoking habit:-
- Tissue (origin): Lung
- Disease: Bronchioalveolar Carcinoma, Non-small cell lung cancer
- Primary/Metastasis: Primary (Collection Site: lung)
- Year of origin: 1981
- Organism:
- Cell line features:
- Cell Type: Club cell
- Morphology: Epithelial-like
- Growth mode: Adherent
- Life span: Infinite (continuous culture)
- Karyotype:-
Doubling time: Approximately 38 hrs - Microsatellite instability: Stable
- Special features:
- Product:-
- Soft agar colony formation assay: yes, colony forming efficiency : 0.83% (in soft agarose)
- Tumorigenicity: yes, produce tumors in athymic nude mice
- Gene expression: SP-A expression (both protein and RNA are derected)
- Sequence variations:
- KRAS (G12C mutation, Oncogenic, Gain-of-function, Heterozygous)
- For information, please visit
- https://depmap.org/portal/cell_line/ACH-000860?tab=mutations
- https://depmap.org/portal/cell_line/ACH-000860?tab=fusions
- KRAS (G12C mutation, Oncogenic, Gain-of-function, Heterozygous)
- Cell Type: Club cell
- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: RPMI-1640 Medium + 10% FBS
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO2, 95% Air
- Temperature: Humidified 37°C incubator
- Subculturing:
- Subculturing method: Enzymatic dissociation
- Cell dissociation solution: 0.25% (w/v) Trypsin-0.53 mM EDTA solution
- Harvesting time: Semi confluent culture (~ 80%)
- Seeding density:-
- Recommended split ratio: 1:3 to 1:6
- Medium Renewal: Every 2 to 3 days
- Cryopreservation:
- Freezing medium: Complete growth medium + 5% DMSO
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial: 1 ml
- Freezing medium: Complete growth medium + 5% DMSO
- Applications:
- Cancer research
- 3D cell culture
- Test system for evaluating cytotoxicity and genotoxicity of chemicals to human lung
- Availability:
- American Type Culture Collection (ATCC): Product link
- UK Health Security Agency Culture Collections: Product link
- American Type Culture Collection (ATCC): Product link
- References
- Images:
- NCI-H358 cell image link (Source: ATCC)
- NCI-H358 cell image link (Source: Culturecollections)
- NCI-H358 cell image link 1 (Source: cytion.com)
- NCI-H358 cell image link 2 (Source: cytion.com)
- NCI-H358 cell image link 3 (Source: cytion.com)
- NCI-H358 cell image link 4 (Source: cytion.com)
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
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